Recombinant Human SGT1/ECD protein (ab211952)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Tags: His-T7 tag N-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human SGT1/ECD protein

  • Purity

    > 90 % SDS-PAGE.
    This protein was expressed in E. coli as inclusion bodies, refolded using unique “temperature shift inclusion body refolding” technology and chromatographically purified.

  • Expression system

    Escherichia coli

  • Accession

    O95905

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MASMTGGQQMGRGHHHHHHENLYFQGGEFELEETMKLATMEDTVEYCLFL IPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGG VPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIE AADFLPKWLDPENSTNRVFFCHGELCIIPAPRKSGAESWLPTTPPTIPQA LNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAHCFLPAGIVAV LKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSVTFTKCLYA QLVQQRFVPDRRSGYRLPPPSDPQYRAHELGMKLAHGFEILCSKCSPHFS DCKKSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERLEMAENYFQLS VDWPESSLAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSP DQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGA ELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDD DLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEP VSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASN LLQSMGVQLPDNTDHRPTSKPTKN

    • Predicted molecular weight

      76 kDa including tags

    • Amino acids

      2 to 644

    • Tags

      His-T7 tag N-Terminus

    • Additional sequence information

      Constructed with 31 aa N-terminal T7 / His / TEV cleavage site Tag. NP_009196.

Specifications

Our Abpromise guarantee covers the use of ab211952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

     This product was previously labelled as SGT1

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituent: 0.32% Tris HCl

    Contains NaCl, KCl, EDTA, Sucrose, DTT.

General Info

  • Alternative names

    • ecd
    • ECD_HUMAN
    • Ecdysoneless homolog
    • hSGT1
    • Human suppressor of GCR two
    • Protein ecdysoneless homolog
    • SGT 1
    • SGT1 protein
    • Suppressor of GCR2
    see all
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab211952? Please let us know so that we can cite the reference in this datasheet.

ab211952 has not yet been referenced specifically in any publications.

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