Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Phosphatase Activity, SDS-PAGE
Product nameRecombinant human SHP1 protein
See all SHP1 proteins and peptides
Biological activitySpecific activity: > 5,000 units/mg of SHP-1. [Unit Definition : One unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate per minute at pH 7.5 at 37°C using 10mM of substrate.]
Purity> 95 % SDS-PAGE.
ab51289 was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceM-GFWEEFES LQKQEVKNLH QRLEGQRPEN KGKNRYKNIL PFDHSRVILQ GRDSNIPGSD YINANYIKNQ LLGPDENAKT YIASQGCLEA TVNDFWQMAW QENSRVIVMT TREVEKGRNK CVPYWPEVGM QRAYGPYSVT NCGEHDTTEY KLRTLQVSPL DNGDLIREIW HYQYLSWPDH GVPSEPGGVL SFLDQINQRQ ESLPHAGPII VHCSAGIGRT GTIIVIDMLM ENISTKGLDC DIDIQKTIQM VRAQRSGMVQ TEAQYKFIYV AIAQFIETTK KKLEVLQSQK GQESEYGNIT Y
Amino acids243 to 541
Our Abpromise guarantee covers the use of ab51289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies).
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.0156% Beta mercaptoethanol, 0.0154% DTT, 0.395% Tris HCl, 0.0292% EDTA, 20% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- EC 18.104.22.168
FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
modificationsPhosphorylated on serine and tyrosine residues.
Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51289 has not yet been referenced specifically in any publications.