Recombinant Human SIGLEC5 protein (Fc Chimera) (ab221413)

Submit a review Submit a question
SDS-PAGE - Recombinant Human SIGLEC5 protein (Fc Chimera) (ab221413)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human SIGLEC5 protein (Fc Chimera)

    • Purity

      > 95 % SDS-PAGE.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      HEK 293 cells

    • Accession

      O15389

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        EKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEI PYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSY FFRVERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSL PGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTNLTCQMK RQGAQVTTERTVQLNVSYAPQTITIFRNGIALEILQNTSYLPVLEGQALR LLCDAPSNPPAHLSWFQGSPALNATPISNTGILELRRVRSAEEGGFTCRA QHPLGFLQIFLNLSVYSLPQLLGPSCSWEAEGLHCRCSFRARPAPSLCWR LEEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLSSDLKVSCKAWNIY GSQSGSVLLLQGRSNLGT

      • Predicted molecular weight

        73 kDa including tags

      • Amino acids

        17 to 434

      • Additional sequence information

        Fused with a human IgG1 Fc tag (Pro 100 - Lys 330; P01857) at the C-terminus (AAH29896).

    Specifications

    Our Abpromise guarantee covers the use of ab221413 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.4
      Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, Sodium chloride, L-Arginine

      Lyophilized from 0.22 µm filtered solution.

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

    General Info

    • Alternative names

      • CD170
      • CD170 antigen
      • CD33 antigen-like 2
      • CD33L2
      • mSiglec-F
      • OB-binding protein 2
      • OB-BP2
      • OBBP2
      • Obesity-binding protein 2
      • Sialic acid binding Ig-like lectin 5
      • Sialic acid binding immunoglobulin-like lectin 5
      • Sialic acid-binding Ig-like lectin 5
      • Sialic acid-binding Ig-like lectin F
      • SIGL5_HUMAN
      • Siglec-5
      • Siglec-F variant
      • Siglec5
      • Siglecf
      see all

    • Function

      Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

    • Tissue specificity

      Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Domain

      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

    • Cellular localization

      Membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human SIGLEC5 protein (Fc Chimera) (ab221413)
      SDS-PAGE - Recombinant Human SIGLEC5 protein (Fc Chimera) (ab221413)

      SDS-PAGE analysis of reduced ab221413 stained overnight with Coomassie Blue.

      The protein migrates as 90-105 kDa under reducing conditions (SDS-PAGE) due to glycosylation.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab221413? Please let us know so that we can cite the reference in this datasheet.

    ab221413 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab221413.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com