Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human SIK1 protein
See all SIK1 proteins and peptides
The specific activity of ab195112 was determined to be 180 nmol/min/mg.
Purity> 90 % Densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceMVIMSEFSAD PAGQGQGQQK PLRVGFYDIE RTLGKGNFAV VKLARHRVTK TQVAIKIIDK TRLDSSNLEK IYREVQLMKL LNHPHIIKLY QVMETKDMLY IVTEFAKNGE MFDYLTSNGH LSENEARKKF WQILSAVEYC HDHHIVHRDL KTENLLLDGN MDIKLADFGF GNFYKSGEPL STWCGSPPYA APEVFEGKEY EGPQLDIWSL GVVLYVLVCG SLPFDGPNLP TLRQRVLEGR FRIPFFMSQD CESLIRRMLV VDPARRITIA QIRQHRWMRA EPCLPGPACP AFSAHSYTSN LGD
Predicted molecular weight61 kDa including tags
Amino acids1 to 303
TagsGST tag N-Terminus
Additional sequence informationNP_775490.2.
Our Abpromise guarantee covers the use of ab195112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204852 (AMARA substrate peptide for SIK + AMPK) can be utilized as a substrate for assessing kinase activity
Previously labelled as Snf1lk.
This product was previously labelled as Snf1lk/SIK-1
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 25% Glycerol (glycerin, glycerine), 0.002% PMSF, 0.004% DTT, 0.003% EDTA, 0.31% Glutathione, 0.87% Sodium chloride, 0.79% Tris HCl
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- myocardial SNF1 like kinase
FunctionTransient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. Potential role in G2/M cell cycle regulation. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus. Translocates to the cytoplasm on phosphorylation where it binds binding to YWHAZ.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab195112 has not yet been referenced specifically in any publications.