Recombinant Human SIRP alpha protein (ab174006)
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Overview
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Product nameRecombinant Human SIRP alpha protein
See all SIRP alpha proteins and peptides -
Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceHEK 293 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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SequenceEEELQVIQPD KSVLVAAGET ATLRCTATSL IPVGPIQWFR GAGPGRELIY NQKEGHFPRV TTVSDLTKRN NMDFSIRIGN ITPADAGTYY CVKFRKGSPD DVEFKSGAGT ELSVRAKPSA PVVSGPAARA TPQHTVSFTC ESHGFSPRDI TLKWFKNGNE LSDFQTNVDP VGESVSYSIH STAKVVLTRE DVHSQVICEV AHVTLQGDPL RGTANLSETI RVPPTLEVTQ QPVRAENQVN VTCQVRKFYP QRLQLTWLEN GNVSRTETAS TVTENKDGTY NWMSWLLVNV SAHRDDVKLT CQVEHDGQPA VSKSHDLKVS AHPKEQGSNT AAENTGSNER
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Molecular weight38 kDa including tags
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Amino acids31 to 370
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TagsHis tag C-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab174006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Endotoxin level< 1.000 Eu/µg
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Purity>95% by SDS-PAGE .
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FormLyophilised
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C.
Information available upon request.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in 100 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
General Info
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Alternative names
- Signal regulatory protein alpha type 1
- Bit
- Brain Ig like molecule with tyrosine based activation motifs
see all -
FunctionImmunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
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Tissue specificityUbiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
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Sequence similaritiesContains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
Cellular localizationMembrane.
- Information by UniProt
Images
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SDS-PAGE - Recombinant Human SIRP alpha protein (ab174006)Reduced SDS-PAGE analysis of ab174006 staining overnight with Coomassie Blue.
DTT-reduced Protein migrates as 45-55 kDa due to glycosylation.
Datasheets and documents
References
ab174006 has not yet been referenced specifically in any publications.
