Recombinant Human SIRP alpha protein (ab174006)

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Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human SIRP alpha protein
    See all SIRP alpha proteins and peptides

  • Biological activity

    Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, His Tag (HPLC-verified) ab174006, Biotinylated Human CD47, Fc,Avitag ab246018, binding reactions. The half maximal inhibitory concentration (IC50) is 0.1245 μg/mL 

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P78324

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      EEELQVIQPD KSVLVAAGET ATLRCTATSL IPVGPIQWFR GAGPGRELIY NQKEGHFPRV TTVSDLTKRN NMDFSIRIGN ITPADAGTYY CVKFRKGSPD DVEFKSGAGT ELSVRAKPSA PVVSGPAARA TPQHTVSFTC ESHGFSPRDI TLKWFKNGNE LSDFQTNVDP VGESVSYSIH STAKVVLTRE DVHSQVICEV AHVTLQGDPL RGTANLSETI RVPPTLEVTQ QPVRAENQVN VTCQVRKFYP QRLQLTWLEN GNVSRTETAS TVTENKDGTY NWMSWLLVNV SAHRDDVKLT CQVEHDGQPA VSKSHDLKVS AHPKEQGSNT AAENTGSNER

    • Predicted molecular weight

      38 kDa including tags

    • Amino acids

      31 to 370

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab174006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C.

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

General Info

  • Alternative names

    • Signal regulatory protein alpha type 1
    • Bit
    • Brain Ig like molecule with tyrosine based activation motifs
    • Brain Ig-like molecule with tyrosine-based activation motifs
    • Brain immunoglobulin like molecule with tyrosine based activation motifs
    • CD172 antigen like family member A
    • CD172 antigen-like family member A
    • CD172a
    • CD172a antigen
    • Inhibitory receptor SHPS-1
    • Macrophage fusion receptor
    • MFR
    • MYD 1
    • Myd 1 antigen
    • MyD-1 antigen
    • p84
    • Protein tyrosine phosphatase non receptor type substrate 1
    • PTPNS1
    • SHP substrate 1
    • SHPS-1
    • SHPS1
    • SHPS1_HUMAN
    • Signal regulatory protein alpha
    • Signal regulatory protein alpha 2
    • Signal regulatory protein alpha 3
    • Signal regulatory protein alpha type 2
    • Signal-regulatory protein alpha-1
    • Signal-regulatory protein alpha-2
    • Signal-regulatory protein alpha-3
    • SIRP
    • Sirp-alpha-1
    • Sirp-alpha-2
    • Sirp-alpha-3
    • SIRPA
    • SIRPalpha
    • SIRPalpha1
    • SIRPalpha2
    • SIRPalpha3
    • Tyrosine phosphatase SHP substrate 1
    • Tyrosine protein phosphatase non receptor type substrate 1
    • Tyrosine-protein phosphatase non-receptor type substrate 1
    see all

  • Function

    Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

  • Tissue specificity

    Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.

  • Sequence similarities

    Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    N-glycosylated.
    Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.

  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human SIRP alpha protein (ab174006)
    SDS-PAGE - Recombinant Human SIRP alpha protein (ab174006)

    Reduced SDS-PAGE analysis of ab174006 staining overnight with Coomassie Blue.

    DTT-reduced Protein migrates as 45-55 kDa due to glycosylation.

  • Biological activity - Recombinant Human SIRP alpha protein (ab174006)
    Biological activity - Recombinant Human SIRP alpha protein (ab174006)

    FACS analysis shows that the binding of Human SIRP alpha, His Tag (HPLC-verified) (ab174006) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing Anti-CD47 antibody. The concentration of SIRP alpha used is 1 μg/mL. IC50=0.2257 μg/mL

  • Biological activity - Recombinant Human SIRP alpha protein (ab174006)
    Biological activity - Recombinant Human SIRP alpha protein (ab174006)

    FACS assay shows that Human SIRP alpha, His Tag (HPLC-verified) (ab174006) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 1 μg/mL

  • Biological activity - Recombinant Human SIRP alpha protein (ab174006)
    Biological activity - Recombinant Human SIRP alpha protein (ab174006)

    Immobilized Human CD47, Fc Tag (HPLC-verified) (ab220587) on AHC Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (ab174006) with an affinity constant of 0.72 μM as determined in BLI assay

  • Biological activity - Recombinant Human SIRP alpha protein (ab174006)
    Biological activity - Recombinant Human SIRP alpha protein (ab174006)

    Loaded Human CD47, Mouse IgG2a Fc Tag, low endotoxin (ab220559) on Protein A Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (ab174006) with an affinity constant of 1.1 μM as determined in BLI assay

  • Biological activity - Recombinant Human SIRP alpha protein (ab174006)
    Biological activity - Recombinant Human SIRP alpha protein (ab174006)

    Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, His Tag (HPLC-verified) ab174006, Biotinylated Human CD47, Fc,Avitag ab246018, binding reactions. The half maximal inhibitory concentration (IC50) is 0.1245 μg/mL 

  • HPLC - Recombinant Human SIRP alpha protein (ab174006)
    HPLC - Recombinant Human SIRP alpha protein (ab174006)

    The purity of Human SIRP alpha, His Tag (HPLC-verified) ab174006 was greater than 90% as determined by SEC-HPLC.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab174006? Please let us know so that we can cite the reference in this datasheet.

ab174006 has not yet been referenced specifically in any publications.

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