Recombinant human SIRT2 protein (ab101132)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human SIRT2 protein
See all SIRT2 proteins and peptides -
Biological activity
Specific Activity: 8000 RLU/min/ng.
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Purity
> 70 % Densitometry.
The purity of SIRT2 was determined to be > 70% by densitometry. Affinity purified. -
Expression system
Baculovirus infected insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
42 kDa including tags -
Amino acids
2 to 352 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab101132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00385% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- FLJ35621
- FLJ37491
- NAD dependent deacetylase sirtuin 2
see all -
Function
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
Tissue specificity
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
Sequence similarities
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
Developmental stage
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
Post-translational
modificationsPhosphorylated at the G2/M transition of the cell cycle. -
Cellular localization
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab101132 has not yet been referenced specifically in any publications.