Key features and details
- Expression system: Baculovirus
- Purity: > 70% Densitometry
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human SIRT2 protein
See all SIRT2 proteins and peptides
Biological activitySpecific Activity: 8000 RLU/min/ng.
Purity> 70 % Densitometry.
The purity of SIRT2 was determined to be > 70% by densitometry
Protein lengthFull length protein
Predicted molecular weight42 kDa including tags
Amino acids2 to 352
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab101132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00385% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- NAD dependent deacetylase sirtuin 2
FunctionNAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
Tissue specificityWidely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
Sequence similaritiesBelongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
Developmental stagePeaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
modificationsPhosphorylated at the G2/M transition of the cell cycle.
Cellular localizationCytoplasm > cytoskeleton. Colocalizes with microtubules.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab101132 has not yet been referenced specifically in any publications.