Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MAEPDPSHPL ETQAGKVQEA QDSDSDSEGG AAGGEADMDF LRNLFSQTLS LGSQKERLLD ELTLEGVARY MQSERCRRVI CLVGAGISTS AGIPDFRSPS TGLYDNLEKY HLPYPEAIFE ISYFKKHPEP FFALAKELYP GQFKPTICHY FMRLLKDKGL LLRCYTQNID TLERIAGLEQ EDLVEAHGTF YTSHCVSASC RHEYPLSWMK EKIFSEVTPK CEDCQSLVKP DIVFFGESLP ARFFSCMQSD FLKVDLLLVM GTSLQVQPFA SLISKAPLST PRLLINKEKA GQSDPFLGMI MGLGGGMDFD SKKAYRDVAW LGECDQGCLA LAELLGWKKE LEDLVRREHA SIDAQSGAGV PNPSTSASPK KSPPPAKDEA RTTEREKPQ
    • Molecular weight

      43 kDa
    • Amino acids

      1 to 389

Specifications

Our Abpromise guarantee covers the use of ab138791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    SPECIFIC ACTIVITY: = 80 units/mg. Activity of ab138791 was tested using the substrate Arg-His-Lys-Lys(Ac)-AFC, which is a fluorogenic, acetylated peptide based on residues 379-382 of p53. UNIT DEFINITION: One unit of the recombinant SIRT2 is defined as the amount of enzyme that deacetylates 1 pmol of substrate Arg-His-Lys-Lys(Ac)-AFC per minute at 37°C in a reaction solution containing 50 mM Tris, pH 7.5, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mM NAD+ and 1 mg/ml BSA.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity

    > 90 % SDS-PAGE.

  • Form

    Liquid
  • Additional notes

    For long term storage, it is recommended to add a carrier protein (0.1% BSA). Avoid multiple freeze/thaw cycles
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.02% Potassium chloride, 0.03% Magnesium chloride, 0.3% Tris, 10% Glycerol, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • FLJ35621
    • FLJ37491
    • NAD dependent deacetylase sirtuin 2
    • NAD-dependent deacetylase sirtuin-2
    • NAD-dependent protein deacetylase sirtuin-2
    • Regulatory protein SIR2 homolog 2
    • Silencing information regulator 2 like
    • Silent information regulator 2
    • SIR2
    • SIR2 like protein 2
    • Sir2 related protein type 2
    • SIR2, S. cerevisiae, homolog-loke 2
    • SIR2-like protein 2
    • SIR2L
    • SIR2L2
    • SIRT2
    • SIRT2_HUMAN
    • Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae)
    • Sirtuin 2
    • Sirtuin type 2
    see all
  • Function

    NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton.
  • Tissue specificity

    Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.
  • Sequence similarities

    Belongs to the sirtuin family.
    Contains 1 deacetylase sirtuin-type domain.
  • Developmental stage

    Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.
  • Post-translational
    modifications

    Phosphorylated at the G2/M transition of the cell cycle.
  • Cellular localization

    Cytoplasm > cytoskeleton. Colocalizes with microtubules.
  • Information by UniProt

Images

  • SIRT2 (ab138791) Activity Assay.

References

ab138791 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab138791.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up