Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human SIRT3 protein
See all SIRT3 proteins and peptides
Biological activityThe specific activity of ab125810 was determined to be 150 RLU/min/mg.
Purity> 70 % Densitometry.
Purity was determined to be >70% by densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Predicted molecular weight66 kDa including tags
Amino acids47 to 399
Our Abpromise guarantee covers the use of ab125810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- hSIRT 3
- Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase
FunctionNAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the sirtuin family. Class I subfamily.
Contains 1 deacetylase sirtuin-type domain.
modificationsProcessed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
Cellular localizationMitochondrion matrix.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125810 has been referenced in 1 publication.
- Nadtochiy SM et al. Potential mechanisms linking SIRT activity and hypoxic 2-hydroxyglutarate generation: no role for direct enzyme (de)acetylation. Biochem J 474:2829-2839 (2017). PubMed: 28673962