Key features and details
- Expression system: Escherichia coli
- Purity: > 85% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE
Product nameRecombinant Human SIRT6 protein
See all SIRT6 proteins and peptides
Purity> 85 % Densitometry.
Purity was determined to be >85% by densitometry.
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight66 kDa including tags
Amino acids1 to 355
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab125578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
- Mono ADP ribosyltransferase sirtuin 6
FunctionNAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain.
Cellular localizationNucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125578 has not yet been referenced specifically in any publications.