Description

  • Product name

    Recombinant Human SLC4A4/NBC protein
  • Purity

    > 90 % SDS-PAGE.
    ab84337 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTE NVEGKPSNLG ERGRARSSTF LRVVQPMFNH SIFTSAVSPA AERIRFILGE EDDSPAPPQL FTELDELLAV DGQEMEWKET ARWIKFEEKV EQGGERWSKP HVATLSLHSL FELRTCMEKG SIMLDREASS LPQLVEMIVD HQIETGLLKP ELKDKVTYTL LRKHRHQTKK SNLRSLADIG KTVSSASRMF TNPDNGSPAM THRNLTSSSL NDISDKPEKD QLKNKFMKKL PRDAEASNVL VGEVDFLDTP FIAFVRLQQA VMLGALTEVP VPTRFLFILL GPKGKAKSYH EIGRAIATLM SDEVFHDIAY KAKDRHDLIA GIDEFLDEVI VLPPGEWDPA IRIEPPKSLP SSDKRKNMYS GGENVQMNGD TPHDGGHGGG GHGDCEELQR TGRFCGGLIK DIKRKAPFFA SDFYDALNIQ
    • Amino acids

      1 to 424

Specifications

Our Abpromise guarantee covers the use of ab84337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Protein previously labeled as SLC4A4.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.00174% PMSF, 0.0077% DTT, 0.316% Tris HCl, 10% Glycerol

General Info

  • Alternative names

    • DKFZp781H1314
    • Electrogenic sodium bicarbonate cotransporter 1
    • hhNMC
    • HNBC 1
    • HNBC1
    • KNBC
    • kNBC 1
    • kNBC1
    • Na(+)/HCO3(-) cotransporter
    • Na+HCO3- cotransporter 4
    • NBC 1
    • NBC 2
    • NBC1
    • NBC2
    • Nbc4
    • NBCE 1
    • NBCE1
    • OTTHUMP00000160355
    • OTTHUMP00000218884
    • OTTHUMP00000218885
    • PNBC
    • S4A4_HUMAN
    • SLC4A4
    • SLC4A5
    • Sodium bicarbonate cotransporter
    • Sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas)
    • Sodium bicarbonate cotransporter kidney
    • sodium bicarbonate cotransporter member 4
    • Sodium bicarbonate cotransporter pancreas
    • Solute carrier family 4 member 4
    • Solute carrier family 4 sodium bicarbonate cotransporter member 4
    • Solute carrier family 4 sodium bicarbonate cotransporter member 5
    • Solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type
    see all
  • Function

    Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.
  • Tissue specificity

    Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.
  • Involvement in disease

    Defects in SLC4A4 are the cause of proximal renal tubular acidosis with ocular abnormalities (pRTA-OA) [MIM:604278]; also known as renal tubular acidosis II. Caused by an impairment of bicarbonate absorption in the proximal tubule, proximal renal tubular acidosis (pRTA) is characterized by a decreased renal HCO3(-) threshold. pRTA-OA is an extremely rare autosomal recessive syndrome characterized by short stature, profound pRTA, mental retardation, bilateral glaucoma, cataracts and bandkeratopathy.
    Note=Loss of interaction with and stimulation by CA4 is the cause of retinitis pigmentosa type 17 (RP17).
  • Sequence similarities

    Belongs to the anion exchanger (TC 2.A.31) family.
  • Post-translational
    modifications

    Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylation of Thr-49 regulates isoform 1 conductance.
    N-glycosylation is not necessary for the transporter basic functions.
  • Cellular localization

    Basolateral cell membrane.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab84337 at approximately 51.5kDa (3µg).

References

ab84337 has not yet been referenced specifically in any publications.

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