Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab84653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol, 0.87% Sodium chloride

General Info

  • Alternative names

    • BSP-1
    • BSP1
    • HsMAD1
    • JV4-1
    • JV41
    • MAD homolog 1
    • MAD mothers against decapentaplegic homolog 1
    • Mad related protein 1
    • Mad-related protein 1
    • MADH1
    • MADR1
    • Mothers against decapentaplegic homolog 1
    • Mothers against DPP homolog 1
    • SMA- AND MAD-RELATED PROTEIN 1
    • SMAD 1
    • SMAD family member 1
    • SMAD mothers against DPP homolog 1
    • Smad1
    • SMAD1_HUMAN
    • TGF beta signaling protein 1
    • Transforming growth factor-beta-signaling protein 1
    see all
  • Function

    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • Tissue specificity

    Ubiquitous. Highest expression seen in the heart and skeletal muscle.
  • Sequence similarities

    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications

    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Information by UniProt

Images

  • SDS-PAGE showing ab84653 at approximately 83kDa.
  • Ab46688 recognizes the tagged recombinant Smad1 protein (ab84653) which has an expected molecular weight of 83 kDa.

References

ab84653 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab84653.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up