Product nameRecombinant Human Smad2 protein
See all Smad2 proteins and peptides
Protein lengthFull length protein
Amino Acid Sequence
SequenceMASMTGGQQMGRGHHHHHHGNLYFQGGEFSSILPFTPPVVKRLLGWKKSA GGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNT KCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKG LPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVET PVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPP PGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPA FWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRN ATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPAT VCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGW GAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS
Molecular weight55 kDa including tags
Amino acids2 to 467
TagsHis-T7 tag N-Terminus
- Anti-Smad2 antibody [5G7] (ab119907)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Smad2 antibody [EP567Y] (ab33875)
- Anti-Smad2 antibody [EP784Y] (ab40855)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Smad2 antibody (ab63576)
- Anti-Smad2 antibody [7A5] (ab71109)
- Anti-6X His tag® antibody (ab9108)
- Anti-T7 tag® antibody (ab9115)
Our Abpromise guarantee covers the use of ab134841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity> 90 % SDS-PAGE.
ab134841 was expressed in E. coli as inclusion bodies, refolded, chromatographically purified and sterile-filtered.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.32% Tris HCl
Note: Buffer also conatins a proprietary formulation of NaCl, KCl, EDTA, Arginine, DTT and Glycerol.
- Drosophila, homolog of, MADR2
FunctionReceptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
Tissue specificityExpressed at high levels in skeletal muscle, heart and placenta.
Sequence similaritiesBelongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
modificationsPhosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
- Information by UniProt
ab134841 has not yet been referenced specifically in any publications.