Recombinant Human Smg1 protein (ab161516)

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Overview

  • Product name

    Recombinant Human Smg1 protein

  • Protein length

    Protein fragment

Description

  • Nature

    Recombinant

  • Source

    Wheat germ
  • Amino Acid Sequence

    • Species

      Human

    • Sequence

      PDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKA VQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLA QLYEGWTAWV

    • Amino acids

      2922 to 3031

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab161516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form

    Liquid

  • Additional notes

    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • 61E3.4
    • ATX
    • hSMG-1
    • hSMG1
    • KIAA0421
    • Lambda interacting protein
    • Lambda-interacting protein
    • Lambda/iota protein kinase C interacting protein
    • Lambda/iota protein kinase C-interacting protein
    • LIP
    • Phosphatidylinositol 3 kinase related kinase
    • Phosphatidylinositol 3 kinase related protein kinase
    • PI 3 kinase related kinase SMG 1
    • Serine/threonine protein kinase SMG1
    • Serine/threonine-protein kinase SMG1
    • SMG-1
    • smg1
    • SMG1_HUMAN
    see all

  • Function

    Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.

  • Tissue specificity

    Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.

  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 1 HEAT repeat.
    Contains 1 PI3K/PI4K domain.

  • Post-translational
    modifications

    Autophosphorylated.

  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Smg1 protein (ab161516)
    SDS-PAGE - Recombinant Human Smg1 protein (ab161516)
    ab161516 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab161516 has not yet been referenced specifically in any publications.

Publishing research using ab161516? Please let us know so that we can cite the reference in this datasheet.

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