Recombinant Human Smoothened protein (ab114221)

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Description

  • Product name

    Recombinant Human Smoothened protein
    See all Smoothened proteins and peptides

  • Expression system

    Wheat germ

  • Accession

    Q99835

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      NLWLVEAEISPELQKRLGRKKKRRKRKKEVCPLAPPPELHPPAPAPSTIP RLPQLPRQKCLVAAGAWGAGDSCRQ GAWTLVSNPFCPEPSPPQDPFLP SAPAPVAWAHGRRQGLGPIHSRTNLMDTELMDADSDF

    • Predicted molecular weight

      40 kDa including tags

    • Amino acids

      653 to 787

Specifications

Our Abpromise guarantee covers the use of ab114221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • Form

    Liquid

  • Additional notes

    This protein is best used within three months from the date of receipt.

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • Frizzled family member 11
    • FZD11
    • Gx
    • Gx protein
    • Protein Gx
    • SMO
    • SMO_HUMAN
    • SMOH
    • Smoothened frizzled family receptor
    • Smoothened homolog
    • Smoothened homolog (Drosophila)
    • Smoothened homolog precursor (SMO) (Gx protein)
    • Smoothened seven transmembrane spanning receptor
    see all

  • Function

    G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.

  • Sequence similarities

    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Smoothened protein (ab114221)
    SDS-PAGE - Recombinant Human Smoothened protein (ab114221)
    ab114221 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

References

ab114221 has not yet been referenced specifically in any publications.

Publishing research using ab114221? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I am wondering how Smoothened antibody gives different band size in Western blot analysis. Predicted band size is 86 kDa but real size is 41 kDa. And I am also wondering whether I can be provided a positive control for this product.

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Answer


The protein fragment is available as ab114221 in our catalog now.
https://www.abcam.com/ab114221.html

It runs at 42 kDa since it is only a fragment not the full length protein.

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