Recombinant Human Smoothened protein (ab114221)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Accession
      Q99835
    • Species
      Human
    • Sequence
      NLWLVEAEISPELQKRLGRKKKRRKRKKEVCPLAPPPELHPPAPAPSTIP RLPQLPRQKCLVAAGAWGAGDSCRQ GAWTLVSNPFCPEPSPPQDPFLP SAPAPVAWAHGRRQGLGPIHSRTNLMDTELMDADSDF
    • Molecular weight
      40 kDa including tags
    • Amino acids
      653 to 787

Associated products

Specifications

Our Abpromise guarantee covers the use of ab114221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.
    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • Frizzled family member 11
    • FZD11
    • Gx
    • Gx protein
    • Protein Gx
    • SMO
    • SMO_HUMAN
    • SMOH
    • Smoothened frizzled family receptor
    • Smoothened homolog
    • Smoothened homolog (Drosophila)
    • Smoothened homolog precursor (SMO) (Gx protein)
    • Smoothened seven transmembrane spanning receptor
    see all
  • Function
    G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.
  • Sequence similarities
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Smoothened protein (ab114221)
    SDS-PAGE - Recombinant Human Smoothened protein (ab114221)
    ab114221 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

References

ab114221 has not yet been referenced specifically in any publications.

Publishing research using ab114221? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

I am wondering how Smoothened antibody gives different band size in Western blot analysis. Predicted band size is 86 kDa but real size is 41 kDa. And I am also wondering whether I can be provided a positive control for this product.


The protein fragment is available as ab114221 in our catalog now.
https://www.abcam.com/ab114221.html

It runs at 42 kDa since it is only a fragment not the full length protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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