Recombinant Human Smoothened protein (ab114221)

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SDS-PAGE - Recombinant Human Smoothened protein (ab114221)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human Smoothened protein
      See all Smoothened proteins and peptides

    • Expression system

      Wheat germ

    • Accession

      Q99835

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        NLWLVEAEISPELQKRLGRKKKRRKRKKEVCPLAPPPELHPPAPAPSTIP RLPQLPRQKCLVAAGAWGAGDSCRQ GAWTLVSNPFCPEPSPPQDPFLP SAPAPVAWAHGRRQGLGPIHSRTNLMDTELMDADSDF

      • Predicted molecular weight

        40 kDa including tags

      • Amino acids

        653 to 787

    Specifications

    Our Abpromise guarantee covers the use of ab114221 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • Frizzled family member 11
      • FZD11
      • Gx
      • Gx protein
      • Protein Gx
      • SMO
      • SMO_HUMAN
      • SMOH
      • Smoothened frizzled family receptor
      • Smoothened homolog
      • Smoothened homolog (Drosophila)
      • Smoothened homolog precursor (SMO) (Gx protein)
      • Smoothened seven transmembrane spanning receptor
      see all

    • Function

      G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.

    • Sequence similarities

      Belongs to the G-protein coupled receptor Fz/Smo family.
      Contains 1 FZ (frizzled) domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Smoothened protein (ab114221)
      SDS-PAGE - Recombinant Human Smoothened protein (ab114221)
      ab114221 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab114221? Please let us know so that we can cite the reference in this datasheet.

    ab114221 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    I am wondering how Smoothened antibody gives different band size in Western blot analysis. Predicted band size is 86 kDa but real size is 41 kDa. And I am also wondering whether I can be provided a positive control for this product.

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    Answer


    The protein fragment is available as ab114221 in our catalog now.
    https://www.abcam.com/ab114221.html

    It runs at 42 kDa since it is only a fragment not the full length protein.

    Read More

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