Recombinant Human SMPDL3A protein (ab185389)

Submit a review Submit a question

Key features and details

  • Expression system: Mammalian
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Human SMPDL3A protein

  • Purity

    > 95 % SDS-PAGE.
    Determined by SEC-HPLC and reducing SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Mammalian

  • Accession

    Q92484

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      LPVAPAGGRNPPPAIGQFWHVTDLHLDPTYHITDDHTKVCASSKGANASN PGPFGDVLCDSPYQLILSAFDFIKNSGQEASFMIWTGDSPPHVPVPELST DTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVA NLWKPWLDEEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLN KTDPANQFEWLESTLNNSQQNKEKVYIIAHVPVGYLPSSQNITAMREYYN EKLIDIFQKYSDVIAGQFYGHTHRDSIMVLSDKKGSPVNSLFVAPAVTPV KSVLEKQTNNPGIRLFQYDPRDYKLLDMLQYYLNLTEANLKGESIWKLEY ILTQTYDIEDLQPESLYGLAKQFTILDSKQFIKYYNYFFVSYDSSVTCDK TCKAFQICAIMNLDNISYADCLKQLYIKHNYVDHHHHHH

    • Predicted molecular weight

      50 kDa including tags

    • Amino acids

      23 to 453

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab185389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    HPLC

  • Form

    Lyophilized

  • Additional notes

    Dissolve the lyophilized protein in 3X PBS.  It is not recommended to reconstitute to a concentration less than 100 µg/ml.  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. After reconstitution aliquot and store at < -20°C.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.

    pH: 7.40
    Constituent: 100% PBS

    Lyophilized from a 0.2 µM filtered solution.

  • Reconstitution
    Dissolve the lyophilized protein in 3X PBS. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. After reconstitution aliquot and store at < -20 degrees, avoid freeze/thaw cycles.

General Info

  • Alternative names

    • Acid sphingomyelinase like phosphodiesterase 3a
    • Acid sphingomyelinase-like phosphodiesterase 3a
    • ASM like phosphodiesterase 3a
    • ASM-like phosphodiesterase 3a
    • ASM3A
    • ASM3A_HUMAN
    • ASML3A
    • FLJ20177
    • SMPDL3A
    • yR36GH4.1
    see all

  • Sequence similarities

    Belongs to the acid sphingomyelinase family.

  • Cellular localization

    Secreted.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab185389? Please let us know so that we can cite the reference in this datasheet.

ab185389 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab185389.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com