Key features and details
- Expression system: Baculovirus
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human SMURF1 protein
See all SMURF1 proteins and peptides
Biological activitySpecific Activity: 0.0055 pmol/min/µg Assay conditions: 50 µl reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and SMURF1 (0-1 µg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against SMURF1, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm).
Purity80 % .
Purity is >80% by SDS-PAGE.
Protein lengthProtein fragment
Predicted molecular weight70 kDa including tags
Amino acids150 to 757
TagsDDDDK tag N-Terminus
Our Abpromise guarantee covers the use of ab95928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C.
Constituents: 0.0462% DTT, 0.395% Tris HCl, 0.05% Tween, 20% Glycerol, 0.58% Sodium chloride
101 µg/ml FLAG peptide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- E3 ubiquitin-protein ligase SMURF1
FunctionE3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 2 WW domains.
modificationsUbiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab95928 has been referenced in 1 publication.
- Wen M et al. Stk38 protein kinase preferentially inhibits TLR9-activated inflammatory responses by promoting MEKK2 ubiquitination in macrophages. Nat Commun 6:7167 (2015). PubMed: 25981615