Recombinant Human SNAP25 protein (ab51048)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P60880
    • Species
      Human
    • Sequence
      MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVML DEQGEQLERIEEGMDQINKD MKEAEKNLTDLGKFCGLCVCPCNKLKSS DAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTND ARENEM DENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEAN QRATKMLGSG
    • Molecular weight
      23 kDa
    • Amino acids
      1 to 206
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab51048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    Recombinant human SNAP25, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0308% DTT, 0.316% Tris HCl, 0.0292% EDTA, 0.29% Sodium chloride

General Info

  • Alternative names
    • bA416N4.2
    • Bdr
    • CMS18
    • dJ1068F16.2
    • FLJ23079
    • HGNC:11132
    • MGC105414
    • MGC139754
    • Resistance to inhibitors of cholinesterase 4 homolog
    • RIC 4
    • RIC4
    • SEC 9
    • SEC9
    • SNAP
    • SNAP 25
    • SNAP-25
    • SNAP-25B
    • SNAP25
    • SNP 25
    • SNP25
    • SNP25_HUMAN
    • sp
    • SUP
    • Super protein
    • Synaptosomal associated 25 kDa protein
    • Synaptosomal associated protein
    • Synaptosomal associated protein 25
    • Synaptosomal associated protein 25kDa
    • Synaptosomal-associated 25 kDa protein
    • Synaptosomal-associated protein 25
    • Synaptosomal-associated protein, 25-KD
    see all
  • Function
    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • Tissue specificity
    Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
  • Sequence similarities
    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • Post-translational
    modifications
    Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
  • Cellular localization
    Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human SNAP25 protein (ab51048)
    SDS-PAGE - Recombinant Human SNAP25 protein (ab51048)

    15% SDS-PAGE. The amount of protein loaded is 3ug.

    Molecular Weight: 25.4 kDa

References

ab51048 has not yet been referenced specifically in any publications.

Publishing research using ab51048? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Could you please help me to find the difference between the following four recombinant proteins SNAP25 you are selling?


Catalog numbers are: ab51048, ab87335, ab155885 and ab74529.

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Answer

I have been in contact with our lab and products team and indeed there is a duplication here which we are currently trying to fix.

I can confirm that ab74529 and ab155885 are identical and ab87335 and ab51048 are also identical.

The difference between ab74529/ab155885 and ab87335/ab51048 is that ab87335/ab51048 is His-tagged whereas ab74529/ab155885 are untagged proteins.

ab87335/ab51048- SNAP25 (Synaptosomal-associated protein) Human, His- tag, Recombinant, E.coli, 25.4 kDa (226 aa), confirmed by MALDI-TOF.
The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters. Recombinant human SNAP25, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

ab74529/ab155885 - SNAP25,1-206aa, Human, Recombinant, E.coli, no tag, 23 kDa (206 aa), confirmed by MALDI-TOF.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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