Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG

Specifications

Our Abpromise guarantee covers the use of ab74529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 90 % SDS-PAGE.
    ab74529 was purified by using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0154% DTT, 0.3025% Tris, 10% Glycerol

General Info

  • Alternative names
    • bA416N4.2
    • Bdr
    • CMS18
    • dJ1068F16.2
    • FLJ23079
    • HGNC:11132
    • MGC105414
    • MGC139754
    • Resistance to inhibitors of cholinesterase 4 homolog
    • RIC 4
    • RIC4
    • SEC 9
    • SEC9
    • SNAP
    • SNAP 25
    • SNAP-25
    • SNAP-25B
    • SNAP25
    • SNP 25
    • SNP25
    • SNP25_HUMAN
    • sp
    • SUP
    • Super protein
    • Synaptosomal associated 25 kDa protein
    • Synaptosomal associated protein
    • Synaptosomal associated protein 25
    • Synaptosomal associated protein 25kDa
    • Synaptosomal-associated 25 kDa protein
    • Synaptosomal-associated protein 25
    • Synaptosomal-associated protein, 25-KD
    see all
  • Function
    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • Tissue specificity
    Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
  • Sequence similarities
    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • Post-translational
    modifications
    Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
  • Cellular localization
    Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab74529 (3μg)
  • Anti-SNAP25 antibody (ab41455) at 1 µg/ml + Recombinant Human SNAP25 protein (ab74529) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 1 minute

References

This product has been referenced in:
  • Sinclair LI & Love S Effect of APOE Genotype on Synaptic Proteins in Earlier Adult Life. J Alzheimers Dis 59:1123-1137 (2017). Read more (PubMed: 28731446) »
  • Stura EA  et al. Structural Framework for Covalent Inhibition of Clostridium botulinum Neurotoxin A by Targeting Cys165. J Biol Chem 287:33607-14 (2012). SDS-PAGE . Read more (PubMed: 22869371) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I have been in contact with our lab and products team and indeed there is a duplication here which we are currently trying to fix.

I can confirm that ab74529 and ab155885 are identical and ab87335 and ab51048 are also identical.

The difference between ab74529/ab155885 and ab87335/ab51048 is that ab87335/ab51048 is His-tagged whereas ab74529/ab155885 are untagged proteins.

ab87335/ab51048- SNAP25 (Synaptosomal-associated protein) Human, His- tag, Recombinant, E.coli, 25.4 kDa (226 aa), confirmed by MALDI-TOF.
The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters. Recombinant human SNAP25, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

ab74529/ab155885 - SNAP25,1-206aa, Human, Recombinant, E.coli, no tag, 23 kDa (206 aa), confirmed by MALDI-TOF.

Read More

Answer


The laboratory said they would remove the EDTA from ab74529 via dialysis.
Alternatively, we have now added the same SNAP25 protein with EDTA removed:
ab155885: https://www.abcam.com/snap25-protein-edta-free-ab155885.html

Read More

Question
Answer

Thank you for contacting us.

I have been in touch with the lab. While they have not run this protein in Western blot, it has been checked in SDS-PAGE and no additional bands have been seen. Would you be willing to provide and image of the western blot and details of your protocol so that we might review these. We do not believe that the addition of more DTT of BME will adversely affect the protein but are unsure whether this will resolve the issue you are facing.

This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link:

https://www.abcam.com/index.html?pageconfig=abpromise

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

Sign up