Recombinant human SOD2/MnSOD protein (ab167988)

Submit a review Submit a question
SDS-PAGE - Recombinant human SOD2/MnSOD protein (ab167988)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant human SOD2/MnSOD protein
      See all SOD2/MnSOD proteins and peptides

    • Biological activity

      Specific activity is > 1,200 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and xanthine oxidase at pH 7.8 at 25°C in a 1.5 ml reaction volume.

    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      P04179

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MKHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK

      • Predicted molecular weight

        24 kDa including tags

      • Amino acids

        25 to 222

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab167988 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 79% Tris HCl, 20% Glycerol

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • Indophenoloxidase B
      • IPO B
      • IPOB
      • Manganese containing superoxide dismutase
      • Manganese SOD
      • Manganese superoxide dismutase
      • Mangano superoxide dismutase
      • Mn SOD
      • Mn superoxide dismutase
      • MNSOD
      • MVCD6
      • SOD 2
      • SOD2
      • SODM_HUMAN
      • Superoxide dismutase [Mn] mitochondrial
      • Superoxide dismutase [Mn], mitochondrial
      • Superoxide dismutase 2 mitochondrial
      see all

    • Function

      Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

    • Involvement in disease

      Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

    • Sequence similarities

      Belongs to the iron/manganese superoxide dismutase family.

    • Post-translational
      modifications

      Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human SOD2/MnSOD protein (ab167988)
      SDS-PAGE - Recombinant human SOD2/MnSOD protein (ab167988)
      15% SDS-PAGE analysis of ab167988 (3 µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab167988? Please let us know so that we can cite the reference in this datasheet.

    ab167988 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab167988.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com