Recombinant human Soluble TNF Receptor I protein (ab9980)

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Key features and details

  • Expression system: Escherichia coli
  • Endotoxin level: < 0.100 Eu/µg
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human Soluble TNF Receptor I protein

  • Endotoxin level

    < 0.100 Eu/µg

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN

    • Predicted molecular weight

      18 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilized

  • Additional notes

    The biological activity of this product was determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L929 cells. The ED50 for this effect, in the presence of 0.25 ng/ml of recombinant human TNF-alpha is 0.05 µg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Please reconstitute this product in 200ul water.

General Info

  • Alternative names

    • CD120a
    • TBP1
    • TNFR1
    • TNFR55
    • TNFRSF1A
    • Tumor necrosis factor receptor 1
    see all

  • Relevance

    TNF RI (CD120a) is a 55 kDa transmembrane glycoprotein that is expressed by virtually all nucleated mammalian cells. Among the numerous cells known to express TNF RI are hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34+ hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF RI. Soluble TNF alpha binds with a Kda in the range of 20-60 pM, while TNF beta binds with a Kda equal to 650 pM. TNF RI relative to TNF RII seems to be the more physiologically relevant receptor, whereas TNF R2 appears to play a direct role in only a limited number of TNF responses. Soluble TNF RI, which blocks TNF alpha activity, has been identified in both urine and blood (1-3 ng/mL). Serum levels of sTNF receptors increase dramatically in certain pathological situations.

  • Cellular localization

    Cytoplasmic

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab9980? Please let us know so that we can cite the reference in this datasheet.

ab9980 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

How is this protein reconstituted?

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Answer

Thank you for your enquiry. Please see the reconstitution protocol below: Centrifuge the vial prior to opening. Reconstitute in 5 mM Tris pH 8.0 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

Good morning, I orderd lyophilized, soluble TNF R1 protein (ab9980) and the datasheet does not indicate what the resuspension buffer is. Will you please send me that information? Thank you,

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Answer

Thank you for your enquiry. This antibody has been lyophilised from 5mM Tris, pH 8.0 and should be reconstituted in dH2O to the desired concentration. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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