Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP
    • Molecular weight
      19 kDa

Specifications

Our Abpromise guarantee covers the use of ab640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of Recombinant Human TNF-α, is 0.125 μg/mL.

  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 98 % SDS-PAGE.
    Sterile filtered. Greater than 98% pure by SDS-PAGE and HPLC analyses.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute by adding 200ul dH2O

General Info

  • Alternative names
    • CD120b
    • p75
    • p75 TNF receptor
    • p75TNFR
    • p80 TNF alpha receptor
    • p80 TNF-alpha receptor
    • Soluble TNFR1B variant 1
    • TBP-2
    • TBPII
    • TNF R II
    • TNF R2
    • TNF R75
    • TNF-R2
    • TNF-RII
    • TNFBR
    • TNFR-II
    • TNFR1B
    • TNFR2
    • TNFR80
    • Tnfrsf1b
    • TNR1B_HUMAN
    • Tumor necrosis factor beta receptor
    • Tumor necrosis factor binding protein 2
    • Tumor necrosis factor receptor 2
    • Tumor necrosis factor receptor superfamily, member 1B
    • Tumor necrosis factor receptor type II
    • Tumor necrosis factor-binding protein 2
    see all
  • Function
    Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
  • Sequence similarities
    Contains 4 TNFR-Cys repeats.
  • Post-translational
    modifications
    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt

References

This product has been referenced in:
  • Dong Y  et al. Essential protective role of tumor necrosis factor receptor 2 in neurodegeneration. Proc Natl Acad Sci U S A 113:12304-12309 (2016). Read more (PubMed: 27791020) »
See 1 Publication for this product

Customer reviews and Q&As

Answer


Ab640 is a fragment of the TNF Receptor II protein. It is comprised of amino acids 33-205 but is an active protein. The activity was determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of Recombinant Human TNF-α, is 0.125 μg/mL. This 172 amino acid protein runs around 19 kDa in a SDS-PAGE gel.


Ab132049, on the other hand, is a recombinant protein comprised of amino acids 23-461 plus a GST tag (25 kDa). The 80 kDa band size is the result of the 438 amino acid protein plus the 25 kDa tag. This protein has not been shown to have activity. It is only validated for ELISA, WB, and SDS-PAGE.


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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