Recombinant human SOX2 protein (ab169843)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human SOX2 protein
    See all SOX2 proteins and peptides

  • Biological activity

    The in vitro function was tested using specific DNA binding assays. 11R proteins were reported to successfully generate induced pluripotent stem (iPS) cells from OG2 MEFs.

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P48431

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMV WSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRAL HMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLG AGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRY DVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASS SPPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQS GPVPGTAINGTLPLSHMESGGGGSPGRRRRRRRRRRR

    • Predicted molecular weight

      37 kDa

    • Amino acids

      1 to 317

Specifications

Our Abpromise guarantee covers the use of ab169843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    The in vitro function was tested using specific DNA binding assays. 11R proteins were reported to successfully generate induced pluripotent stem (iPS) cells from OG2 MEFs.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.50
    Constituents: Potassium chloride, 0.24% Tris, EDTA, Glycerol, Sodium chloride

    Also contains DTT and Arginine.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • ANOP3
    • cb236
    • Delta EF2a
    • lcc
    • MCOPS3
    • MGC148683
    • MGC2413
    • RGD1565646
    • Sex determining region Y box 2
    • SOX 2
    • Sox2
    • SOX2_HUMAN
    • SRY (sex determining region Y) box 2
    • SRY box containing gene 2
    • SRY related HMG box 2
    • SRY related HMG box gene 2
    • SRY-box 2
    • Transcription factor SOX 2
    • Transcription factor SOX-2
    • ysb
    see all

  • Function

    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.

  • Involvement in disease

    Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.

  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.

  • Post-translational
    modifications

    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab169843? Please let us know so that we can cite the reference in this datasheet.

ab169843 has been referenced in 1 publication.

  • Liu Z & Kraus WL Catalytic-Independent Functions of PARP-1 Determine Sox2 Pioneer Activity at Intractable Genomic Loci. Mol Cell 65:589-603.e9 (2017). PubMed: 28212747

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