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    recombinant-human-spak-protein-ab107696.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Recombinant human SPAK protein (ab107696)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant human SPAK protein (ab107696)
  • Functional Studies - Recombinant human SPAK protein (ab107696)
  • SDS-PAGE - Recombinant human SPAK protein (ab107696)
  • Functional Studies - Recombinant human SPAK protein (ab107696)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human SPAK protein
  • Biological activity

    Specific activity: 22 nmol/min/mg
  • Purity

    > 95 % SDS-PAGE.
    Purification determined by densitometry
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    Q9UEW8
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      63 kDa including tags
    • Amino acids

      1 to 545
    • Tags

      His tag N-Terminus

Associated products

  • Related Products

    • Anti-SPAK antibody (ab106936)
    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-SPAK antibody (ab79045)
  • Substrate reagent

    • Native Pig Myelin Basic Protein (ab64311)

Specifications

Our Abpromise guarantee covers the use of ab107696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

     This product was previously labelled as STK39

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00385% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • DCHT
    • DKFZp686K05124
    • OTTHUMP00000165175
    • PASK
    • Proline alanine rich STE20 related kinase
    • Serine threonine kinase 39
    • Serine threonine kinase 39 (STE20/SPS1 homolog yeast)
    • Serine/threonine protein kinase 39
    • Serine/threonine-protein kinase 39
    • Small intestine SPAK like kinase
    • SPAK
    • Ste 20 related kinase
    • Ste-20-related kinase
    • Ste20 like protein kinase
    • STE20/SPS1 homolog
    • STE20/SPS1 related proline alanine rich protein kinase
    • STE20/SPS1-related proline-alanine-rich protein kinase
    • STK 39
    • Stk39
    • STK39_HUMAN
    see all
  • Function

    May act as a mediator of stress-activated signals.
  • Tissue specificity

    Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • Domain

    PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus when caspase-cleaved.
  • Target information above from: UniProt accession Q9UEW8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant human SPAK protein (ab107696)
    SDS-PAGE - Recombinant human SPAK protein (ab107696)
    SDS PAGE analysis of ab107696
  • Functional Studies - Recombinant human SPAK protein (ab107696)
    Functional Studies - Recombinant human SPAK protein (ab107696)

    The specific activity of SPAK (ab107696) was determined to be 1.9 nmol/min/mg as per activity assay protocol.

  • SDS-PAGE - Recombinant human SPAK protein (ab107696)
    SDS-PAGE - Recombinant human SPAK protein (ab107696)
    SDS Page analysis of ab107696
  • Functional Studies - Recombinant human SPAK protein (ab107696)
    Functional Studies - Recombinant human SPAK protein (ab107696)
    ab107696 specific activity determined to be 22 nmol/min/mg

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab107696? Please let us know so that we can cite the reference in this datasheet.

ab107696 has not yet been referenced specifically in any publications.

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