Recombinant human SPAK protein (ab107696)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
-
Product name
Recombinant human SPAK protein -
Biological activity
Specific activity: 22 nmol/min/mg -
Purity
> 95 % SDS-PAGE.
Purification determined by densitometry -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
63 kDa including tags -
Amino acids
1 to 545 -
Tags
His tag N-Terminus
-
Associated products
-
Related Products
-
Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab107696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Functional Studies
-
Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as STK39
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00385% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- DCHT
- DKFZp686K05124
- OTTHUMP00000165175
see all -
Function
May act as a mediator of stress-activated signals. -
Tissue specificity
Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
Domain
PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins. -
Cellular localization
Cytoplasm. Nucleus. Nucleus when caspase-cleaved. - Information by UniProt
Images
-
SDS-PAGE - Recombinant human SPAK protein (ab107696)SDS PAGE analysis of ab107696
-
Functional Studies - Recombinant human SPAK protein (ab107696)The specific activity of SPAK (ab107696) was determined to be 1.9 nmol/min/mg as per activity assay protocol.
-
SDS-PAGE - Recombinant human SPAK protein (ab107696)SDS Page analysis of ab107696 -
Functional Studies - Recombinant human SPAK protein (ab107696)ab107696 specific activity determined to be 22 nmol/min/mg
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab107696 has not yet been referenced specifically in any publications.