Description

  • Product name

    Recombinant human SPAM1 protein
    See all SPAM1 proteins and peptides
  • Biological activity

    The activity of ab174000 is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >40,000 U/mg. Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH 5.35  at 37 °C  in a 2.0 ml reaction mixture.

  • Purity

    > 92 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      LNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATG QGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYM PVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEAT EKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYN GSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREA IRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASG IVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQE QGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKF YCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFY
    • Predicted molecular weight

      52 kDa including tags
    • Amino acids

      36 to 482
    • Tags

      His tag C-Terminus
    • Additional sequence information

      GenBank: AAH26163.1

Specifications

Our Abpromise guarantee covers the use of ab174000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

     This product was previously labelled as Hyaluronidase PH20. The protein migrates as 64-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Reconstitute for long term storage.

    Information available upon request.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • epididymis secretory sperm binding protein Li 96n
    • HEL-S-96n
    • HYA1
    • HYAL PH-20
    • HYAL PH20
    • Hyal-PH20
    • HYAL1
    • HYAL3
    • HYAL5
    • HYALP_HUMAN
    • Hyaluronidase PH-20
    • Hyaluronoglucosaminidase PH-20
    • MGC26532
    • PH-20 Hyaluronidase
    • PH20
    • PH20 Hyaluronidase
    • SPAG15
    • SPAM-1
    • Spam1
    • Sperm adhesion molecule 1
    • sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
    • Sperm surface protein PH-20
    • Sperm surface protein PH20
    • zona pellucida binding
    see all
  • Function

    Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
  • Tissue specificity

    Testis.
  • Sequence similarities

    Belongs to the glycosyl hydrolase 56 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt

Images

  • SDS-PAGE analysis of reduced ab174000 stained overnight with Coomassie Blue.

    DTT-reduced Protein migrates as 64-66 kDa due to glycosylation.

References

ab174000 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab174000.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up