Recombinant Human SPAM1 protein (Tagged) (ab114725)

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Description

  • Product name

    Recombinant Human SPAM1 protein (Tagged)
    See all SPAM1 proteins and peptides

  • Expression system

    Wheat germ

  • Accession

    P38567

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      RSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSS DYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKE

    • Predicted molecular weight

      37 kDa including tags

    • Amino acids

      346 to 445

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab114725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • Form

    Liquid

  • Additional notes

    This product was previously labelled as Hyaluronidase PH20.

    Protein concentration is above or equal to 0.05 ug/ul.

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • epididymis secretory sperm binding protein Li 96n
    • HEL-S-96n
    • HYA1
    • HYAL PH-20
    • HYAL PH20
    • Hyal-PH20
    • HYAL1
    • HYAL3
    • HYAL5
    • HYALP_HUMAN
    • Hyaluronidase PH-20
    • Hyaluronoglucosaminidase PH-20
    • MGC26532
    • PH-20 Hyaluronidase
    • PH20
    • PH20 Hyaluronidase
    • SPAG15
    • SPAM-1
    • Spam1
    • Sperm adhesion molecule 1
    • sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
    • Sperm surface protein PH-20
    • Sperm surface protein PH20
    • zona pellucida binding
    see all

  • Function

    Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

  • Tissue specificity

    Testis.

  • Sequence similarities

    Belongs to the glycosyl hydrolase 56 family.

  • Post-translational
    modifications

    N-glycosylated.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human SPAM1 protein (ab114725)
    SDS-PAGE - Recombinant Human SPAM1 protein (ab114725)

    12.5% SDS-PAGE analysis of ab114725 stained with Coomassie Blue.

References

ab114725 has not yet been referenced specifically in any publications.

Publishing research using ab114725? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Product code: 112304
Inquiry: I will like to know if is possible that you send me hyaluronidase PH-20 to use in a research project in my group

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Answer

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Question

concentration de ab114725 lot 1384718 GR98907-1 ?
0.05 mg/ml comme indiqué sur la fiche technique ou 0.1 mg/ml comme indiqué sur le tube?

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Answer

Merci de nous avoir contactés.

Le laboratoire vient de me confirmer que la concentration de la protéineHyaluronidase PH20, lot GR98907-1 est de 0.1 mg/ml.

La concentration de cette protéine dépend du lot, la fiche technique va être mise à jour avec cette information dans les meilleurs délais.

Nous sommes désolés pour la confusion et espérons que cette information vous sera utile. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Question

functional activity?

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Answer

Thank you for contacting us and sorry for the delay of our reply.

I just received a reply from the source of the Hyaluronidase PH20 protein ab114725. They confirmed that this protein has not been tested for functional studies.
All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

If you would like to test the protein in this untested application, please contact our Scientific Support team prior to the purchase by replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

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