Key features and details
- Expression system: Escherichia coli
- Suitable for: WB, ELISA, SDS-PAGE, MS
Product nameRecombinant Human SPARC protein
See all SPARC proteins and peptides
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab92965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesAfter reconstitution, store in working aliquots at -80ºC.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.316% Tris HCl, 0.0292% EDTA, 1.16% Sodium chloride, 0.342% Maltose
- Basement membrane protein 40
- Basement-membrane protein 40
FunctionAppears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Sequence similaritiesBelongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Developmental stageExpressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab92965 has not yet been referenced specifically in any publications.