Description

  • Product name

    Recombinant Human SPARC protein
    See all SPARC proteins and peptides
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab92965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

    Mass Spectrometry

  • Form

    Lyophilised
  • Additional notes

    After reconstitution, store in working aliquots at -80ºC.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: 0.316% Tris HCl, 0.0292% EDTA, 1.16% Sodium chloride, 0.342% Maltose

General Info

  • Alternative names

    • AA517111
    • Basement membrane protein 40
    • Basement-membrane protein 40
    • BM 40
    • BM-40
    • BM40
    • Cysteine rich protein
    • hm:zeh0062
    • MGC128090
    • OI17
    • ON
    • Osteonectin
    • Secreted acidic cystein rich glycoprotein
    • Secreted protein acidic and cysteine rich
    • Secreted protein acidic and rich in cysteine
    • Secreted protein acidic cysteine rich
    • Secreted protein acidic cysteine rich (osteonectin)
    • SPARC
    • SPRC
    • SPRC_HUMAN
    see all
  • Function

    Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
  • Sequence similarities

    Belongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • Developmental stage

    Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
  • Information by UniProt

Images

  • ab92965 analysed by 10% SDS-PAGE stained with Coomassie Blue (CB) and immunoblotted with anti-MBP (WB).

References

ab92965 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us and for your patience.

I had to contact the laboratory in order to get more information about the use of these three proteins.

I can confirm, that for the ab92965 SPARC protein (MBP) to our knowledge it has not been tested for injection in mice or other animals (besides it uses as immunogen for polyclonal antibodies production in rabbits intramuscular). It could be that it will work for injection in mice but we can not guarantee this and we have no information about different administration routes (intravenous, intramuscular etc.).

The two other proteins have been used in mice before. See the following links:

1. ab9571 VEGF protein (Active)

http://www.ncbi.nlm.nih.gov/pubmed/19088027

http://www.ncbi.nlm.nih.gov/pubmed/10784613

http://www.ncbi.nlm.nih.gov/pubmed/11689883

http://www.ncbi.nlm.nih.gov/pubmed/12036930

http://www.ncbi.nlm.nih.gov/pubmed/9401980

http://www.ncbi.nlm.nih.gov/pubmed/15358628

http://www.ncbi.nlm.nih.gov/pubmed/16477043

http://www.ncbi.nlm.nih.gov/pubmed/16888632

http://www.pnas.org/content/104/8/2837.full

http://www.ncbi.nlm.nih.gov/pubmed/18180379

http://www.ncbi.nlm.nih.gov/pubmed/15067321

http://www.ncbi.nlm.nih.gov/pubmed/20056792

http://www.ncbi.nlm.nih.gov/pubmed/22025303

http://www.pnas.org/content/early/2011/07/25/1016546108.abstract



2. ab50036 TGF beta 1 protein (Active)

http://www.ncbi.nlm.nih.gov/pubmed/20304823

http://www.ncbi.nlm.nih.gov/pubmed/22761317

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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