Recombinant Human SPF45 protein (ab174402)

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SDS-PAGE - Recombinant Human SPF45 protein (ab174402)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human SPF45 protein

    • Purity

      > 90 % SDS-PAGE.
      ab174402 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      Q96I25

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMSLYDDLGVETSDSKTEGWSKNFKLLQSQL QVKKAALTQAKSQRTKQSTVLAPVIDLKRGGSSDDRQIVDTPPHVAAGLK DPVPSGFSAGEVLIPLADEYDPMFPNDYEKVVKRQREERQRQRELERQKE IEEREKRRKDRHEASGFARRPDPDSDEDEDYERERRKRSMGGAAIAPPTS LVEKDKELPRDFPYEEDSRPRSQSSKAAIPPPVYEEQDRPRSPTGPSNSF LANMGGTVAHKIMQKYGFREGQGLGKHEQGLSTALSVEKTSKRGGKIIVG DATEKDASKKSDSNPLTEILKCPTKVVLLRNMVGAGEVDEDLEVETKEEC EKYGKVGKCVIFEIPGAPDDEAVRIFLEFERVESAIKAVVDLNGRYFGGR VVKACFYNLDKFRVLDLAEQV

      • Predicted molecular weight

        47 kDa including tags

      • Amino acids

        1 to 401

      • Tags

        His tag N-Terminus

      • Additional sequence information

        NCBI Accession No. NP_116294.

    Specifications

    Our Abpromise guarantee covers the use of ab174402 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid

    • Additional notes

       This product was previously labelled as RBM17

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 0.88% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.02% DTT

    General Info

    • Alternative names

      • 45 kDa-splicing factor
      • RBM 17
      • Rbm17
      • RNA binding motif protein 17
      • RNA-binding motif protein 17
      • SPF 45
      • SPF45
      • SPF45_HUMAN
      • Splicing factor (45 kD)
      • Splicing factor (45kD)
      • Splicing factor 45
      • Splicing factor 45 kDa
      • Splicing factor 45kDa
      see all

    • Function

      Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.

    • Sequence similarities

      Contains 1 G-patch domain.
      Contains 1 RRM (RNA recognition motif) domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human SPF45 protein (ab174402)
      SDS-PAGE - Recombinant Human SPF45 protein (ab174402)

      15% SDS-PAGE analysis of ab174402 (3μg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab174402? Please let us know so that we can cite the reference in this datasheet.

    ab174402 has not yet been referenced specifically in any publications.

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