Recombinant Human SPF45 protein (ab174402)

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Description

  • Product name

    Recombinant Human SPF45 protein

  • Purity

    > 90 % SDS-PAGE.
    ab174402 is purified using conventional chromatography techniques.

  • Expression system

    Escherichia coli

  • Accession

    Q96I25

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMSLYDDLGVETSDSKTEGWSKNFKLLQSQL QVKKAALTQAKSQRTKQSTVLAPVIDLKRGGSSDDRQIVDTPPHVAAGLK DPVPSGFSAGEVLIPLADEYDPMFPNDYEKVVKRQREERQRQRELERQKE IEEREKRRKDRHEASGFARRPDPDSDEDEDYERERRKRSMGGAAIAPPTS LVEKDKELPRDFPYEEDSRPRSQSSKAAIPPPVYEEQDRPRSPTGPSNSF LANMGGTVAHKIMQKYGFREGQGLGKHEQGLSTALSVEKTSKRGGKIIVG DATEKDASKKSDSNPLTEILKCPTKVVLLRNMVGAGEVDEDLEVETKEEC EKYGKVGKCVIFEIPGAPDDEAVRIFLEFERVESAIKAVVDLNGRYFGGR VVKACFYNLDKFRVLDLAEQV

    • Predicted molecular weight

      47 kDa including tags

    • Amino acids

      1 to 401

    • Tags

      His tag N-Terminus

    • Additional sequence information

      NCBI Accession No. NP_116294.

Specifications

Our Abpromise guarantee covers the use of ab174402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid

  • Additional notes

     This product was previously labelled as RBM17

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names

    • 45 kDa-splicing factor
    • RBM 17
    • Rbm17
    • RNA binding motif protein 17
    • RNA-binding motif protein 17
    • SPF 45
    • SPF45
    • SPF45_HUMAN
    • Splicing factor (45 kD)
    • Splicing factor (45kD)
    • Splicing factor 45
    • Splicing factor 45 kDa
    • Splicing factor 45kDa
    see all

  • Function

    Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.

  • Sequence similarities

    Contains 1 G-patch domain.
    Contains 1 RRM (RNA recognition motif) domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human SPF45 protein (ab174402)
    SDS-PAGE - Recombinant Human SPF45 protein (ab174402)

    15% SDS-PAGE analysis of ab174402 (3μg).

References

ab174402 has not yet been referenced specifically in any publications.

Publishing research using ab174402? Please let us know so that we can cite the reference in this datasheet.

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