Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant human SPHK1 protein
See all SPHK1 proteins and peptides
Biological activityThe specific activity of ab125662 was determined to be 450 - 515 nmol/min/mg.
Purity> 70 % Densitometry.
Purity was determined to be >70% by densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight71 kDa including tags
Amino acids1 to 384
Our Abpromise guarantee covers the use of ab125662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- SK 1
- Sphingosine kinase 1
FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Tissue specificityWidely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
Sequence similaritiesContains 1 DAGKc domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125662 has not yet been referenced specifically in any publications.