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Recombinant Human SREBP2 protein (ab114215)

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SDS-PAGE - Recombinant Human SREBP2 protein (ab114215)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human SREBP2 protein
      See all SREBP2 proteins and peptides
    • Expression system

      Wheat germ
    • Accession

      Q12772
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        QAFCKNLLERAIESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDS VGVMSPPLSRSSVLKSALGPDIICRWWTSAITVAISWLQGDDAAVRSHFT
      • Predicted molecular weight

        37 kDa including tags
      • Amino acids

        801 to 900

    Specifications

    Our Abpromise guarantee covers the use of ab114215 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

      Western blot

    • Form

      Liquid
    • Additional notes

       

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • AI608257
      • bHLHd2
      • Class D basic helix-loop-helix protein 2
      • OTTHUMP00000028740
      • Processed sterol regulatory element-binding protein 2
      • SRBP2_HUMAN
      • SREBF 2
      • Srebf2
      • SREBF2 protein
      • SREBP 2
      • SREBP-2
      • SREBP2
      • SREBP2gc
      • sterol regulatory element binding factor 2
      • Sterol regulatory element binding protein 2
      • Sterol regulatory element binding transcription factor 2
      • Sterol regulatory element-binding transcription factor 2
      see all
    • Function

      Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
    • Tissue specificity

      Ubiquitously expressed in adult and fetal tissues.
    • Sequence similarities

      Belongs to the SREBP family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      modifications

      At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
    • Cellular localization

      Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
    • Target information above from: UniProt accession Q12772 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human SREBP2 protein (ab114215)
      SDS-PAGE - Recombinant Human SREBP2 protein (ab114215)
      ab114215 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab114215? Please let us know so that we can cite the reference in this datasheet.

    ab114215 has not yet been referenced specifically in any publications.

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