Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant human STK25 protein
See all STK25 proteins and peptides
Biological activityThe specific activity of ab125588 was determined to be 11 nmol/min/mg.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight76 kDa including tags
Amino acids1 to 426
Our Abpromise guarantee covers the use of ab125588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Serine/threonine kinase 25
- Serine/threonine kinase 25 (STE20 homolog, yeast)
FunctionOxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.
Tissue specificityUbiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Golgi apparatus. Localizes to the Golgi apparatus.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125588 has not yet been referenced specifically in any publications.