Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 46% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human STK33 protein (Active)
See all STK33 proteins and peptides
Biological activitySpecific Activity: ≥10 pmol/min/µgAssay Conditions: Assay was performed using ADP-Glo reagents in Kinase buffer containing 2 mM DTT using MBP substrate (0.1 mg/ml) and 100 μM ATP. Reaction was performed at 30°C for 40 mins.
Purity>= 46 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight84 kDa including tags
Amino acids2 to 514
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab271749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.64% Sodium chloride, 20% Glycerol (glycerin, glycerine), 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.63% Tris HCl, 0.04% Tween, 0.02% Potassium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Hypothetical protein FLJ35932
- Serine threonine kinase 33
- Serine/threonine kinase 33
Tissue specificityHighly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271749 has not yet been referenced specifically in any publications.