Overview

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected insect cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      ADP MEQPPAP KSKLKKLSED SLTKQPEEVF DVLEKLGEGS YGSVFKAIHK ESGQV VAIKQ VPVESDLQEI IKEISIMQQC DSPYVVKYYG SYFKNTDLWI VMEYCGAGSV SDIIRLRNKT LIEDEIATIL KSTLKGLEYL HFMRKIHRDI K AGNILLNTE GHAKLADFGV AGQLTDTMAK RNTVIGTPFW MAPEVIQEIG YNCVADIWSL GITSIEMAEG KPPYADIHPM RAIFMIPTNP PPTFRKPELW SDDFTDFVKK CLVKNPEQRA TATQLLQHPF IKNAKPVS IL RDLITEAMEI KAKRHEEQQR ELEEEEENSD EDELD HHHHHH
    • Molecular weight

      38 kDa
    • Amino acids

      1 to 322
    • Tags

      His tag C-Terminus
    • Additional sequence information

      36kDa subunit (NP_006272).

Specifications

Our Abpromise guarantee covers the use of ab219219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 90 % SDS-PAGE.
    ab219219 was purified using conventional chromatography techniques.
  • Form

    Liquid
  • Additional notes

    Previously labelled as STK3. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.4
    Constituents: 90% PBS, 10% Glycerol

General Info

  • Alternative names

    • 0610042I06Rik
    • EC 2.7.11.1
    • FLJ90748
    • KB 1458E12.1
    • Kinase responsive to stress 1
    • KRS1
    • Mammalian STE20 like protein kinase 2
    • Mammalian STE20-like protein kinase 2
    • Mammalian sterile 20-like 2
    • Mess1
    • MST
    • MST-2
    • MST2
    • Mst3
    • Serine/threonine kinase 3
    • Serine/threonine kinase 3 (STE20 homolog, yeast)
    • Serine/threonine kinase 3 (Ste20, yeast homolog)
    • Serine/threonine protein kinase 3
    • Serine/threonine protein kinase Krs1
    • Serine/threonine-protein kinase 3
    • Serine/threonine-protein kinase Krs-1
    • STE20 like kinase MST2
    • STE20-like kinase MST2
    • Stk3
    • STK3_HUMAN
    • wu:fc19e11
    • zgc:55383
    see all
  • Function

    Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.
  • Tissue specificity

    Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SARAH domain.
  • Cellular localization

    Cytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab219219 (3μg).

    Molecular weight: 40-57kDa (SDS-PAGE under reducing conditions).

References

ab219219 has not yet been referenced specifically in any publications.

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