Recombinant Human Stromal interaction molecule 1 protein (ab78681)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Stromal interaction molecule 1 protein
See all Stromal interaction molecule 1 proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab78681 is purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SMLSHSHSEK ATGTSSGANS EESTAAEFCR IDKPLCHSED EKLSFEAVRN IHKLMDDDAN GDVDVEESDE FLREDLNYHD PTVKHSTFHG EDKLISVEDL WKAWKSSEVY NWTVDEVVQW LITYVELPQY EETFRKLQLS GHAMPRLAVT NTTMTGTVLK MTDRSHRQKL QLKALDTVLF GPPLLTRHNH LKD
underlined sequence is the CaM tag. -
Amino acids
23 to 213
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Specifications
Our Abpromise guarantee covers the use of ab78681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.50
Constituent: 0.242% Tris
General Info
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Alternative names
- D11S4896E
- GOK
- OTTHUMP00000164512
see all -
Function
Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. -
Tissue specificity
Ubiquitously expressed in various human primary cells and tumor cell lines. -
Involvement in disease
Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition. -
Sequence similarities
Contains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain. -
Domain
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
Post-translational
modificationsGlycosylation is required for cell surface expression.
Phosphorylated predominantly on Ser residues. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules. - Information by UniProt
Images
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15% SDS-PAGE showing ab68681 at approximately 38kDa (3µg).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab78681 has not yet been referenced specifically in any publications.