Question (56148) | Recombinant human Sumo 1 protein (ab3801)

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Question

Hi there,
I have a question about your product, SUMO-1 (Active) protein, used for in vitro SUMOyation assays. When it says "active", does this mean that it has been converted into the mature form and therefore, can be used in sumoylation processes?
Because, physiologically, in order for SUMO-1 to bind to the target, it has to first become activated by a SENP member, and if we use the inactive form, it might not conjugate.
Is this right?
Thank you,

Answer

Thank you very much for contacting us with your question.

We have a couple of SUMO1 proteins; are you interested in ab3801? This protein only contains the first 97 amino acids, and amino acids 98-101comprise the propeptide region that is cleaved upon activation by SENP1 or SENP2-

http://www.uniprot.org/uniprot/P63165

So, the protein ab3801 is the mature, active form of SUMO1 and can be used in functional assays.

I hope that this information will be useful, but if you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

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