Recombinant Human Sumo 2 protein (ab140420)
- Datasheet
- References
- Protocols
Overview
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Product name
Recombinant Human Sumo 2 protein
See all Sumo 2 proteins and peptides -
Protein length
Full length protein
Description
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Nature
Recombinant -
Source
Escherichia coli -
Amino Acid Sequence
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Accession
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Species
Human -
Sequence
MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCER QGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG -
Molecular weight
18 kDa including tags -
Amino acids
1 to 93 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab140420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Purity
> 95 % Densitometry. -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 0.70
Preservative: 1.02% Imidazole
Constituents: 0.002% PMSF, 0.71% Sodium phosphate, 0.004% DTT, 25% Glycerol, 1.75% Sodium chloride
General Info
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Alternative names
- HSMT 3
- HSMT3
- MGC117191
see all -
Function
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. -
Tissue specificity
Broadly expressed. -
Sequence similarities
Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain. -
Post-translational
modificationsPolymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4.
Cleavage of precursor form by SENP1 or SENP2 is necessary for function. -
Cellular localization
Nucleus. Nuclear bodies. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Sumo 2 protein (ab140420)SDS-PAGE analysis of ab140420.
References
ab140420 has not yet been referenced specifically in any publications.
