Recombinant human Sumo 3 protein (ab3802)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Suitable for: WB, Functional Studies
Description
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Product name
Recombinant human Sumo 3 protein
See all Sumo 3 proteins and peptides -
Biological activity
The final fraction of enzyme contains a single polypeptide band of 11 kDa. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
11 kDa
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab3802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
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Form
Liquid -
Additional notes
The final fraction of enzyme contains a single polypeptide band of 11 kDa. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.50
Preservative: 0.68% Imidazole
Constituents: 0.0087% PMSF, 0.0154% DTT, 0.158% Tris HCl, 10% Glycerol, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Small ubiquitin like modifier 3
- Small ubiquitin related modifier 3
- Small ubiquitin-related modifier 3
see all -
Function
Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. -
Tissue specificity
Expressed predominantly in liver. -
Sequence similarities
Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain. -
Post-translational
modificationsPolymeric chains can be formed through Lys-11 cross-linking.
Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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Functional Studies - Recombinant human Sumo 3 protein (ab3802)
Sumoylation assay
Left Lane: Topo I protein (Topo I fragment: ab3828)
Middle Lane: Sumo 1 sumoylated Topo I (Sumo 1: ab3801; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)
Right Lane: Sumo 3 sumoylated Topo I (Sumo 3: ab3802; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)
Note: Topo I is S35-Met labeled.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab3802 has not yet been referenced specifically in any publications.
