Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Molecular weight
      11 kDa

Specifications

Our Abpromise guarantee covers the use of ab3802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The final fraction of enzyme contains a single polypeptide band of 11 kDa.
  • Applications

    Western blot

    Functional Studies

  • Form
    Liquid
  • Additional notes
    The final fraction of enzyme contains a single polypeptide band of 11 kDa.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.50
    Preservative: 0.68% Imidazole
    Constituents: 0.0087% PMSF, 0.0154% DTT, 0.158% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Small ubiquitin like modifier 3
    • Small ubiquitin related modifier 3
    • Small ubiquitin-related modifier 3
    • SMT3 homolog 1
    • SMT3 suppressor of mif two 3 homolog 1
    • SMT3 suppressor of mif two 3 homolog 3
    • SMT3, yeast, homolog 1
    • SMT3A
    • Smt3B
    • SMT3H1
    • SUMO-2
    • SUMO-3
    • sumo3
    • SUMO3_HUMAN
    • Ubiquitin like protein SMT3A
    • Ubiquitin-like protein SMT3B
    see all
  • Function
    Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.
  • Tissue specificity
    Expressed predominantly in liver.
  • Sequence similarities
    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications
    Polymeric chains can be formed through Lys-11 cross-linking.
    Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human Sumo 3 protein (ab3802)
    Functional Studies - Recombinant human Sumo 3 protein (ab3802)
    Sumoylation assay

    Left Lane: Topo I protein (Topo I fragment: ab3828)

    Middle Lane: Sumo 1 sumoylated Topo I (Sumo 1: ab3801; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)

    Right Lane: Sumo 3 sumoylated Topo I (Sumo 3: ab3802; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)

    Note: Topo I is S35-Met labeled.

References

ab3802 has not yet been referenced specifically in any publications.

Publishing research using ab3802? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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