Recombinant human Sumo 3 protein (ab3802)
- Datasheet
- References
- Protocols
Overview
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Product nameRecombinant human Sumo 3 protein
See all Sumo 3 proteins and peptides -
Protein lengthFull length protein
Description
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NatureRecombinant
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SourceEscherichia coli
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Amino Acid Sequence
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SpeciesHuman
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Molecular weight11 kDa
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab3802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activityThe final fraction of enzyme contains a single polypeptide band of 11 kDa.
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Applications
Western blot
Functional Studies
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FormLiquid
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Additional notesThe final fraction of enzyme contains a single polypeptide band of 11 kDa.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.50
Preservative: 0.68% Imidazole
Constituents: 0.0087% PMSF, 0.0154% DTT, 0.158% Tris HCl, 10% Glycerol, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Small ubiquitin like modifier 3
- Small ubiquitin related modifier 3
- Small ubiquitin-related modifier 3
see all -
FunctionUbiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.
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Tissue specificityExpressed predominantly in liver.
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Sequence similaritiesBelongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain. -
Post-translational
modificationsPolymeric chains can be formed through Lys-11 cross-linking.
Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function. -
Cellular localizationCytoplasm.
- Information by UniProt
Images
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Functional Studies - Recombinant human Sumo 3 protein (ab3802)Sumoylation assayLeft Lane: Topo I protein (Topo I fragment: ab3828)
Middle Lane: Sumo 1 sumoylated Topo I (Sumo 1: ab3801; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)
Right Lane: Sumo 3 sumoylated Topo I (Sumo 3: ab3802; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)
Note: Topo I is S35-Met labeled.
References
ab3802 has not yet been referenced specifically in any publications.
