Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, HPLC
Product nameRecombinant Human Surfactant protein D/SP-D
See all Surfactant protein D/SP-D proteins and peptides
Purity> 95 % SDS-PAGE.
The purity of ab152069 is greater than 95%, as determined by SEC-HPLC and reducing SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthFull length protein
SequenceAGMKTYSHRTMPSACTLVMCSSVESGLPGRDGRDGREGPRGEKGDPGLPG AAGQAGMPGQAGPVGPKGDNGSVGEPGPKGDTGPSGPPGPPGVPGPAGRE GPLGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSAGARGLAGPKGERGV PGERGVPGNTGAAGSAGAMGPQGSPGARGPPGLKGDKGIPGDKGAKGESG LPDVASLRQQVEALQGQVQHLQAAFSQYKKVELFPNGQSVGEKIFKTAGF VKPFTEAQLLCTQAGGQLASPRSAAENAALQQLVVAKNEAAFLSMTDSKT EGKFTYPTGESLVYSNWAPGKPNDDGGSEDCVEIFTNGKWNDRACGEKRL VVCEFVDHHHHHH
Predicted molecular weight37 kDa including tags
Amino acids21 to 375
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab152069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Previously labelled as Surfactant protein D.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride
ReconstitutionAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 µg/ml.
Dissolve the lyophilized protein in 1X PBS.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- COLEC 7
FunctionContributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Sequence similaritiesBelongs to the SFTPD family.
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain.
modificationsThe N-terminus is blocked.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
Cellular localizationSecreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab152069 has been referenced in 1 publication.
- Wong SSW et al. Fungal melanin stimulates surfactant protein D-mediated opsonization of and host immune response to Aspergillus fumigatus spores. J Biol Chem 293:4901-4912 (2018). PubMed: 29414772