Key features and details
- Expression system: Escherichia coli
- Purity: >= 80% SDS-PAGE
- Endotoxin level: < 1.000 Eu/mg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human Surfactant protein D/SP-D
See all Surfactant protein D/SP-D proteins and peptides
Purity>= 80 % SDS-PAGE.
Endotoxin level< 1.000 Eu/mg
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMGSSHHHHHH SSGLVPRGSH MGSVASLRQQ VEALQGQVQH LQAAFSQYKK VELFPNGQSV GEKIFKTAGF VKPFTEAQLL CTQAGGQLAS PRSAAENAAL QQLVVAKNEA AFLSMTDSKT EGKFTYPTGE SLVYSNWAPG EPNDDGGSED CVEIFTNGKW NDRACGEKRL VVCEF
Predicted molecular weight19 kDa
Molecular weight information18.9 kDa (175 aa, 224-375 aa + His Tag), confirmed by MALDI-TOF.
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab285831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is manufactured by BioVision, an Abcam company and was previously called 7328 SFTPD, human recombinant. 7328-100 is the same size as the 100 µg size of ab285831.
SFTPD, as known as Surfactant pulmonary-associated protein D, is a member of the collectin family of C-type lectins that is synthesized in many tissues including respiratory epithelial cells in the lung, and contains one C-type lectin domain and one collagen-like domain. It is humoral molecules of the innate immune system, and is considered a functional candidate in chronic periodontitis. Besides it is involved in the development of acute and chronic inflammation of the lung. Several human lung diseases are characterized by decreased levels of bronchoalveolar SFTPD. Recombinant human SFTPD protein, fused to His-tag at N-terminus, was expressed in E.coli.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.315% Tris HCl, 2.402% Urea, 10% Glycerol (glycerin, glycerine)
- COLEC 7
FunctionContributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Sequence similaritiesBelongs to the SFTPD family.
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain.
modificationsThe N-terminus is blocked.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
Cellular localizationSecreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab285831 has not yet been referenced specifically in any publications.