Description

  • Product name

    Recombinant Human SWAP70 protein
  • Expression system

    Wheat germ
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSLKEELLKAIWHAFTALDQDHSGKVSKSQLKVLSHNLCTVLKVPHDPV ALEEHFRDDDEGPVSNQGYMPYLNRFILEKVQDNFDKIEFNRMCWTLCVK KNLTKNPLLITEEDAFKIWVIFNFLSEDKYPLIIVSEEIEYLLKKLTEAM GGGWQQEQFEHYKINFDDSKNGLSAWELIELIGNGQFSKGMDRQTVSMAI NEVFNELILDVLKQGYMMKKGHRRKNWTERWFVLKPNIISYYVSEDLKDK KGDILLDENCCVESLPDKDGKKCLFLVKCFDKTFEISASDKKKKQEWIQA IHSTIHLLKLGSPPPHKEARQRRKELRKKQLAEQEELERQMKELQAANES KQQELEAVRKKLEEAASRAAEEEKKRLQTQVELQARFSTELEREKLIRQQ MEEQVAQKSSELEQYLQRVRELEDMYLKLQEALEDERQARQDEETVRKLQ ARLLEEESSKRAELEKWHLEQQQAIQTTEAEKQELENQRVLKEQALQEAM EQLEQLELERKQALEQYEEVKKKLEMATNKTKSWKDKVAHHEGLIRLIEP GSKNPHLITNWGPAAFTEAELEEREKNWKEKKTTE
    • Predicted molecular weight

      95 kDa including tags
    • Amino acids

      1 to 585

Associated products

Specifications

Our Abpromise guarantee covers the use of ab131864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • KIAA0640
    • 70kDa
    • AV235546
    • FLJ39540
    • HSPC321
    • SWA 70 protein
    • SWAP 70
    • SWAP switching B-cell complex 70kDa subunit
    • SWAP-70
    • SWAP70
    • Switch associated protein 70
    • Switch-associated protein 70
    • SWP70_HUMAN
    see all
  • Function

    Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
  • Tissue specificity

    Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760).
  • Sequence similarities

    Contains 1 PH domain.
  • Domain

    The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
  • Post-translational
    modifications

    Tyrosine-phosphorylated.
  • Cellular localization

    Cytoplasm. Cell membrane. Nucleus. Cell projection > lamellipodium. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia.
  • Information by UniProt

Images

  • 12.5% SDS-PAGE analysis of ab131864 stained with Coomassie Blue.

References

ab131864 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131864.
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