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Recombinant human Syk protein (ab60886)

  • Datasheet
Submit a review Submit a question References (1)

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Functional Studies - Recombinant human Syk protein (ab60886)
  • SDS-PAGE - Recombinant human Syk protein (ab60886)
  • SDS-PAGE - Recombinant human Syk protein (ab60886)
  • Functional Studies - Recombinant human Syk protein (ab60886)
  • SDS-PAGE - Recombinant human Syk protein (ab60886)
  • Western blot - Recombinant human Syk protein (ab60886)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: SDS-PAGE, Functional Studies, WB

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Description

  • Product name

    Recombinant human Syk protein
    See all Syk proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Tags

      GST tag N-Terminus

Associated products

  • Related Products

    • Anti-Syk antibody (ab15528)
    • Anti-Syk antibody [SYK-01] (ab3993)
    • Anti-Syk antibody [EP573Y] (ab40781)
    • Anti-Syk (phospho Y348) antibody (ab52212)
  • Substrate reagent

    • Poly (Glu, Tyr) peptide (ab204877)

Specifications

Our Abpromise guarantee covers the use of ab60886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Form

    Liquid
  • Additional notes

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • EC 2.7.10.2
    • kinase Syk
    • KSYK
    • KSYK_HUMAN
    • p72-Syk
    • p72syk
    • Spleen tyrosine kinase
    • Syk
    • Tyrosine protein kinase SYK
    • Tyrosine-protein kinase SYK
    see all
  • Function

    Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen.
  • Tissue specificity

    Widely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro-B-cells to plasma cells.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.
  • Domain

    The SH2 domains mediate the interaction of SYK with the phosphorylated ITAM domains of transmembrane proteins. Some proteins like CLEC1B have a partial ITAM domain (also called hemITAM) containing a single YxxL motif. The interaction with SYK requires CLEC1B homodimerization.
  • Post-translational
    modifications

    Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation.
    Autophosphorylated. Phosphorylated on tyrosine residues by LYN following receptors engagement. Phosphorylation on Tyr-323 creates a binding site for CBL, an adapter protein that serves as a negative regulator of BCR-stimulated calcium ion signaling. Phosphorylation at Tyr-348 creates a binding site for VAV1. Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3-kinase-independent pathway (By similarity). Phosphorylation on Ser-297 is very common, it peaks 5 minutes after BCR stimulation, and creates a binding site for YWHAG. Phosphorylation at Tyr-630 creates a binding site for BLNK. Dephosphorylated by PTPN6.
  • Cellular localization

    Cell membrane. Cytoplasm, cytosol.
  • Target information above from: UniProt accession P43405 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human Syk protein (ab60886)
    Functional Studies - Recombinant human Syk protein (ab60886)
    The specific activity of Syk (ab60886) was determined to be 103 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human Syk protein (ab60886)
    SDS-PAGE - Recombinant human Syk protein (ab60886)
    SDS PAGE analysis of ab60886
  • SDS-PAGE - Recombinant human Syk protein (ab60886)
    SDS-PAGE - Recombinant human Syk protein (ab60886)
    SDS PAGE analysis of ab60886
  • Functional Studies - Recombinant human Syk protein (ab60886)
    Functional Studies - Recombinant human Syk protein (ab60886)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human Syk protein (ab60886)
    SDS-PAGE - Recombinant human Syk protein (ab60886)
    ab60886 on SDS-PAGE, MW ~100 kDa.
  • Western blot - Recombinant human Syk protein (ab60886)
    Western blot - Recombinant human Syk protein (ab60886)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab60886? Please let us know so that we can cite the reference in this datasheet.

    ab60886 has been referenced in 1 publication.

    • Wang X  et al. Discovery of a Natural Syk Inhibitor from Chinese Medicine through a Docking-Based Virtual Screening and Biological Assay Study. Molecules 23:N/A (2018). PubMed: 30487406

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