Recombinant Human Synaptotagmin V protein (ab171492)

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SDS-PAGE - Recombinant Human Synaptotagmin V protein (ab171492)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human Synaptotagmin V protein
      See all Synaptotagmin V proteins and peptides

    • Purity

      > 90 % SDS-PAGE.
      ab171492 was purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      O00445

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSLGRLQYS LDYDFQSGQL LVGILQAMGL AALDLGGSSD PYVRVYLLPD KRRRYETKVH RQTLNPHFGE TFAFKVPYVE LGGRVLVMAV YDFDRFSRND AIGEVRVPMS SVDLGRPVQA WRELQAAPRE EQEKLGDICF SLRYVPTAGK LTVIVLEAKN LKKMDVGGLS DPYVKVHLLQ GGKKVRKKKT TIKKNTLNPY YNEAFSFEVP CDQVQKVQVE LTVLDYDKLG KNEAIGRVAV GAAAGGAGLR HWADMLANPR RPIAQWHSLR PPDRVRLLPA P

      • Predicted molecular weight

        34 kDa including tags

      • Amino acids

        109 to 386

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab171492 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.03% DTT, 0.32% Tris HCl, 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride

    General Info

    • Alternative names

      • Synaptotagmin 5
      • Synaptotagmin IX
      • Synaptotagmin V
      • Synaptotagmin-5
      • Syt5
      • SYT5_HUMAN
      • SytV
      see all

    • Function

      May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface.

    • Sequence similarities

      Belongs to the synaptotagmin family.
      Contains 2 C2 domains.

    • Cellular localization

      Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Recycling endosome membrane. In mast cells, localizes to the endocytic recycling compartment.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Synaptotagmin V protein (ab171492)
      SDS-PAGE - Recombinant Human Synaptotagmin V protein (ab171492)
      15% SDS-PAGE analysis of 3µg ab171492.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab171492? Please let us know so that we can cite the reference in this datasheet.

    ab171492 has not yet been referenced specifically in any publications.

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