Recombinant Human TAF1 protein (Tagged) (ab198119)

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SDS-PAGE - Recombinant Human TAF1 protein (Tagged) (ab198119)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: GST tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human TAF1 protein (Tagged)
      See all TAF1 proteins and peptides

    • Purity

      > 90 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      P21675-2

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLLDDDDQVAFSFILDNIVT QKMMAVPDSWPFHHPVNKKFVPDYYKVIVNPMDLETIRKNISKHKYQSRE SFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEK DICTAKEAALEEAE

      • Predicted molecular weight

        42 kDa including tags

      • Amino acids

        1519 to 1651

      • Tags

        GST tag N-Terminus

      • Additional sequence information

        GenBank Accession No. NM_004606

    Specifications

    Our Abpromise guarantee covers the use of ab198119 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Store at -80°C.

      pH: 8.00
      Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol

    General Info

    • Alternative names

      • BA2R
      • CCG 1
      • CCG1
      • CCGS
      • Cell cycle G1 phase defect
      • Cell cycle gene 1 protein
      • Complementation of cell cycle block G1 to S
      • DYT3 protein
      • NSCL 2
      • NSCL2
      • OF
      • p250
      • TAF 1
      • TAF(II)250
      • TAF1 RNA polymerase II
      • TAF130
      • TAF145
      • TAF2A
      • TAFII 250
      • TAFII250
      • TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD
      • TATA box binding protein (TBP)-associated factor
      • TBP associated factor 1
      • TBP associated factor 145 kDa
      • TBP associated factor 250 kDa
      • Transcription factor TFIID p250 polypeptide
      • Transcription initiation factor TFIID 250 kDa subunit
      • Transcription initiation factor TFIID subunit 1
      • YGR274C
      see all

    • Relevance

      TAF1 functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains.

    • Cellular localization

      Nuclear

    Images

    • SDS-PAGE - Recombinant Human TAF1 protein (Tagged) (ab198119)
      SDS-PAGE - Recombinant Human TAF1 protein (Tagged) (ab198119)

      4-20% SDS-PAGE stained with Coomassie Blue.
      Lane 1: ab198119 (2.7 µg)
      Lane 2: Protein Marker

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab198119? Please let us know so that we can cite the reference in this datasheet.

    ab198119 has not yet been referenced specifically in any publications.

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