The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Bioactivity: Measured by ab155627 ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been precoated with 50 µl/well of ab155627 solution at 8-30 µg/ml.
< 1.000 Eu/µg
>95% by SDS-PAGE . Lyophilized from 0.22 µm filtered solution.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage
Axonal glycoprotein TAG 1
Axonal glycoprotein TAG-1
Axonal glycoprotein TAG1
contactin 2 (axonal)
Transient axonal glycoprotein 1
Transiently expressed axonal glycoprotein
May play a role in the initial growth and guidance of axons. May be involved in cell adhesion.
Belongs to the immunoglobulin superfamily. Contactin family. Contains 4 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Cell membrane. Attached to the neuronal membrane by a GPI-anchor and is also released from neurons.