Recombinant human TAK1 protein (ab132950)
Key features and details
- Expression system: Wheat germ
- Purity: >= 80% Purified via GST Tag
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB, ELISA
Description
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Product name
Recombinant human TAK1 protein -
Purity
>= 80 % Purified via GST Tag. -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSTASAASSSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVV CKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPV CLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPK ALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEV FEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPP LIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQY PCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLES KLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIA ATTGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPTR SHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHC KMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDE NKSLSTYYQQCKKQLEVIRSQQQKRQGTS -
Predicted molecular weight
89 kDa including tags -
Amino acids
1 to 579
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab132950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Western blot
ELISA
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Form
Liquid -
Additional notes
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- M3K7_HUMAN
- MAP3K 7
- Map3k7
see all -
Function
Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAssociation with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP. - Information by UniProt
Images
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12.5% SDS-PAGE analysis of ab132950 stained with Coomassie Blue.
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Kinase assay using ab132950 and [gamma 32P] ATP.
Fig 1: SDS-PAGE gel
Fig 2: Autoradiograph of SDS-PAGE gelKinase substrates:
H3 histone, family 3A - calf thymus - Molecular weight: approx. 16.5-17 kDa on SDS-PAGE
Myelin basic protein - bovine brain - Molecular weight: approx. 21-22 kDa on SDS-PAGE
Histone H1.0 - calf thymus - Molecular weight: approx. 32-33 kDa on SDS-PAGE
Beta-casein protein - bovine milk - Molecular weight: approx. 33-34 kDa on SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab132950 has not yet been referenced specifically in any publications.