Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human Tankyrase protein
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETE QITLDVLADMGHEELKEIGINAYGHRHKLIKGVERLLGGQQGTNPYLTFH CVNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRI QKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDER HAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQML FCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQA YPEYLITYQIMKPEAPSQTATAAEQKT
Predicted molecular weight63 kDa including tags
Amino acids1001 to 1327
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab198639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.48% Tris buffer, 0.64% Sodium chloride, 0.017% Potassium chloride, 20% Glycerol, 0.05% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ADP-ribosyltransferase diphtheria toxin-like 5
FunctionMay regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TERF1, and thereby contribute to the regulation of telomere length.
Tissue specificityUbiquitous; highest levels in testis.
Sequence similaritiesContains 15 ANK repeats.
Contains 1 PARP catalytic domain.
Contains 1 SAM (sterile alpha motif) domain.
modificationsPhosphorylated on serine residues by MAPK kinases upon insulin stimulation.
Cellular localizationCytoplasm. Golgi apparatus membrane. Chromosome > centromere. Nucleus > nuclear pore complex. Chromosome > telomere. Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198639 has not yet been referenced specifically in any publications.