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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription TFIIB
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Recombinant Human TATA binding protein TBP (ab81897)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Western blot - Recombinant Human TATA binding protein TBP (ab81897)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: GSA, SDS-PAGE, WB

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    Description

    • Product name

      Recombinant Human TATA binding protein TBP
      See all TATA binding protein TBP proteins and peptides
    • Biological activity

      1 unit equals 1 nanogram of purified protein.
    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Escherichia coli
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQ NTNSL SILEEQQRQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQAVAA AAV QQSTSQ QATQGTSGQA PQLFHSQTLT TAPLPGTTPL YPSPMTPMTP ITPATPASES SG IVPQLQN IVSTVNLGCK LDLKTIALRA RNAEYNPKRF AAVIMRIREP RTTALIFSSG K MVCTGAKS EEQSRLAARK YARVVQKLGF PAKFLDFKIQ NMVGSCDVKF PIRLEGLVLT HQQFSSYEP ELFPGLIYRM IKPRIVLLIF VSGKVVLTGA KVRAEIYEAF ENIYPILKG FRKTT
      • Predicted molecular weight

        39 kDa
      • Amino acids

        1 to 339
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-TATA binding protein TBP antibody (ab28175)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (ab63766)
      • Anti-TATA binding protein TBP antibody - Loading Control (ab74222)

    Specifications

    Our Abpromise guarantee covers the use of ab81897 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Gel Supershift Assays

      SDS-PAGE

      Western blot

      EMSA

    • Form

      Liquid
    • Additional notes

      1 unit equals 1 nanogram of purified protein.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.9
      Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • GTF2D
      • GTF2D1
      • HDL4
      • MGC117320
      • MGC126054
      • MGC126055
      • SCA17
      • TATA binding factor
      • TATA box factor
      • TATA sequence binding protein
      • TATA sequence-binding protein
      • TATA-binding factor
      • TATA-box binding protein N-terminal domain
      • TATA-box factor
      • TATA-box-binding protein
      • TBP
      • TBP_HUMAN
      • TFIID
      • Transcription initiation factor TFIID TBP subunit
      see all
    • Function

      General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
    • Tissue specificity

      Widely expressed, with levels highest in the testis and ovary.
    • Involvement in disease

      Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
    • Sequence similarities

      Belongs to the TBP family.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P20226 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Recombinant Human TATA binding protein TBP (ab81897)
      Western blot - Recombinant Human TATA binding protein TBP (ab81897)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab81897? Please let us know so that we can cite the reference in this datasheet.

    ab81897 has been referenced in 1 publication.

    • Grabherr MG  et al. Exploiting nucleotide composition to engineer promoters. PLoS One 6:e20136 (2011). PubMed: 21625601

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Dear technical support: This customer has highly interested in ab81897 (TATA binding protein TBP (His tag)), this customer wants to know the molecular weight of this antibody, could you please help this customer to check this information? Thanks for your kindly assistance. Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Oct 04 2011

    Answer

    Thank you for contacting us. ab81897 is a recombinant full length human TATA binding protein TBP with a His tag. The SwissProt ID for this protein is P20226 with a size of 37,7 kDa reported. With the his tag included this size increases to 39.4 kDa. This can be seen in the Western blot image presented on the datasheet. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 04 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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