Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMELGSCL EGGREAAEEE GEPEVKKRRL LCVEFASVAS CDAAVAQCFL AENDWEMERA LNSYFEPPVE ESALERRPET ISEPKTYVDL TNEETTDSTT SKISPSEDTQ QENGSMFSLI TWNIDGLDLN NLSERARGVC SYLALYSPDV IFLQEVIPPY YSYLKKRSSN YEIITGHEEG YFTAIMLKKS RVKLKSQEII PFPSTKMMRN LLCVHVNVSG NELCLMTSHL ESTRGHAAER MNQLKMVLKK MQEAPESATV IFAGDTNLRD REVTRCGGLP NNIVDVWEFL GKPKHCQYTW DTQMNSNLGI TAACKLRFDR IFFRAAAEEG HIIPRSLDLL GLEKLDCGRF PSDHWGLLCN LDIIL
    • Molecular weight

      43 kDa including tags
    • Amino acids

      1 to 362
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab140722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF
  • Purity

    > 85 % SDS-PAGE.
    ab140722 is purified using conventional chromatography techniques.
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names

    • 5'-Tyr-DNA phosphodiesterase
    • 5'-tyrosyl-DNA phosphodiesterase
    • AD 022
    • AD022
    • dJ30M3.3
    • EAP 2
    • EAP II
    • EAP2
    • EAPII
    • ETS 1 associated protein 2
    • ETS 1 associated protein II
    • ETS1 associated protein 2
    • ETS1 associated protein II
    • ETS1-associated protein 2
    • ETS1-associated protein II
    • MGC111021
    • MGC9099
    • RP1-30M3.3
    • tdp2
    • TRAF and TNF receptor associated protein
    • TRAF and TNF receptor-associated protein
    • TTRAP
    • TYDP2_HUMAN
    • Tyr DNA phosphodiesterase 2
    • Tyr-DNA phosphodiesterase 2
    • Tyrosyl DNA phosphodiesterase 2
    • Tyrosyl-DNA phosphodiesterase 2
    see all
  • Function

    DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the CCR4/nocturin family.
  • Cellular localization

    Nucleus. Nucleus > PML body.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab140722 (3 µg).

References

ab140722 has not yet been referenced specifically in any publications.

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