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Recombinant Human TDP43 protein (ab140718)

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SDS-PAGE - Recombinant Human TDP43 protein (ab140718)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human TDP43 protein
      See all TDP43 proteins and peptides

    • Purity

      > 85 % SDS-PAGE.
      ab140718 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      Q13148

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSEY IRVTEDENDE PIEIPSEDDG TVLLSTVTAQ FPGACGLRYR NPVSQCMRGV RLVEGILHAP DAGWGNLVYV VNYPKDNKRK MDETDASSAV KVKRAVQKTS DLIVLGLPWK TTEQDLKEYF STFGEVLMVQ VKKDLKTGHS KGFGFVRFTE YETQVKVMSQ RHMIDGRWCD CKLPNSKQSQ DEPLRSRKVF VGRCTEDMTE DELREFFSQY GDVMDVFIPK PFRAFAFVTF ADDQIAQSLC GEDLIIKGIS VHISNA

      • Predicted molecular weight

        34 kDa including tags

      • Amino acids

        1 to 260

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab140718 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • ALS10
      • OTTHUMP00000002171
      • OTTHUMP00000002172
      • OTTHUMP00000002173
      • TADBP_HUMAN
      • TAR DNA binding protein
      • TAR DNA binding protein 43
      • TAR DNA-binding protein 43
      • TARDBP
      • TDP 43
      • TDP-43
      • TDP43
      see all

    • Function

      DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.

    • Tissue specificity

      Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.

    • Involvement in disease

      Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.

    • Sequence similarities

      Contains 2 RRM (RNA recognition motif) domains.

    • Domain

      The RRM domains can bind to both DNA and RNA.

    • Post-translational
      modifications

      Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
      Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
      Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.

    • Cellular localization

      Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human TDP43 protein (ab140718)
      SDS-PAGE - Recombinant Human TDP43 protein (ab140718)
      15% SDS-PAGE analysis of ab140718 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab140718? Please let us know so that we can cite the reference in this datasheet.

    ab140718 has been referenced in 1 publication.

    • Ormeño F  et al. Chaperone Mediated Autophagy Degrades TDP-43 Protein and Is Affected by TDP-43 Aggregation. Front Mol Neurosci 13:19 (2020). PubMed: 32132902

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