Recombinant Human Testin protein (ab172155)

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SDS-PAGE - Recombinant Human Testin protein (ab172155)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 0.100 Eu/µg
    • Tags: His tag C-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human Testin protein
      See all Testin proteins and peptides

    • Purity

      > 95 % SDS-PAGE.

    • Endotoxin level

      < 0.100 Eu/µg

    • Expression system

      Escherichia coli

    • Accession

      Q9UGI8-1

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MDLENKVKKMGLGHEQGFGAPCLKCKEKCEGFELHFWRKICRNCKCGQEE HDVLLSNEEDRKVGKLFEDTKYTTLIAKLKSDGIPMYKRNVMILTNPVAA KKNVSINTVTYEWAPPVQNQALARQYMQMLPKEKQPVAGSEGAQYRKKQL AKQLPAHDQDPSKCHELSPREVKEMEQFVKKYKSEALGVGDVKLPCEMDA QGPKQMNIPGGDRSTPAAVGAMEDKSAEHKRTQYSCYCCKLSMKEGDPAI YAERAGYDKLWHPACFVCSTCHELLVDMIYFWKNEKLYCGRHYCDSEKPR CAGCDELIFSNEYTQAENQNWHLKHFCCFDCDSILAGEIYVMVNDKPVCK PCYVKNHAVVCQGCHNAIDPEVQRVTYNNFSWHASTECFLCSCCSKCLIG QKFMPVEGMVFCSVECKKRMS

      • Predicted molecular weight

        48 kDa

      • Amino acids

        1 to 421

      • Tags

        His tag C-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab172155 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.20
      Constituents: 0.02% DTT, 0.88% Sodium chloride, 99% PBS

    • Reconstitution
      Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS.

    General Info

    • Alternative names

      • DKFZP586B2022
      • MGC1146
      • TES
      • TES_HUMAN
      • TESS
      • TESS 2
      • TESS2
      • Testin
      • Testis derived transcript
      • Testis derived transcript (3 LIM domains)
      see all

    • Function

      Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth.

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the prickle / espinas / testin family.
      Contains 3 LIM zinc-binding domains.
      Contains 1 PET domain.

    • Domain

      The N-terminal and the C-terminal halves of the protein can associate with each other, thereby hindering interactions with ZYX.

    • Cellular localization

      Cytoplasm. Cell junction > focal adhesion. Detected along actin stress fibers.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Testin protein (ab172155)
      SDS-PAGE - Recombinant Human Testin protein (ab172155)

      SDS-PAGE analysis of ab172155 under  Non reducing (Lane 1) or reducing conditions (Lane 2)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab172155? Please let us know so that we can cite the reference in this datasheet.

    ab172155 has not yet been referenced specifically in any publications.

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