Description

  • Product name

    Recombinant Human TFIIF protein
  • Biological activity

    1 unit equals 1 nanogram of purified protein.
  • Purity

    > 95 % SDS-PAGE.
    Recombinant RAP74 protein is isolated from an E. coli strain that carries the coding sequence of the human RAP74 subunit of TFIIF under the control of T7 promoter; recombinant RAP30 protein is isolated from an E. coli strain that carries the coding sequence of the human RAP30 subunit of TFIIF under the control of T7 promoter; TFIIF is reconstituted by combining equimolar quantities of recombinant RAP30 and RAP74.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab81891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    EMSA

  • Form

    Liquid
  • Additional notes

    1 unit equals 1 nanogram of purified protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol

General Info

  • Alternative names

    • ATP dependent helicase GTF2F2
    • BTF 4
    • BTF4
    • General transcription factor IIF polypeptide 1
    • General transcription factor IIF polypeptide 1 74 kDa subunit protein
    • General transcription factor IIF polypeptide 1 74kDa
    • General Transcription Factor IIF Polypeptide 2
    • General transcription factor IIF polypeptide 2 30kDa
    • GTF2F1
    • GTF2F1 protein
    • GTF2F2
    • RAP 30
    • RAP 74
    • RAP30
    • RAP74
    • TF2F 1
    • TF2F 2
    • TF2F1
    • TF2F2
    • TFIIF alpha
    • TFIIF beta
    • Transcription initiation factor IIF alpha subunit
    • Transcription Initiation Factor IIF beta Subunit
    • Transcription initiation factor IIF subunit beta
    • Transcription initiation factor RAP30
    • Transcription initiation factor RAP74
    see all
  • Relevance

    The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
  • Cellular localization

    Nuclear

References

ab81891 has not yet been referenced specifically in any publications.

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